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Table 3 DNA sequence analysis of gpt mutations obtained from the lung of B[a]P-instilled and control mice

From: Alterations in the mutagenicity and mutation spectrum induced by benzo[a]pyrene instilled in the lungs of gpt delta mice of various ages

Type of mutation Nucleo-tide # Sequence Change Amino acid change Number
Control B[a]P
Months Months
3d 11 24 3e 11 24
Base substitution             
Transition             
G:C → A:T 26 tGg → tAg   Trp → Stop    1   2a 1
  27 tgG → tgA   Trp → Stop    1   1  
  37 Cag → Tag   Gln → Stop    1   2 1
  58 Gca → Aca CpG Ala → Thr    1    
  64 Cga → Tga CpG Arg → Stop 1 5b 1   5b 1
  86 tGg → tAg   Trp → Stop   3b   1   
  87 tgG → tgA   Trp → Stop   2a     
  92 gGc → gAc   Gly → Asp   2a     
  110 cGt → cAt CpG Arg → His 5c 4 3b   4b 6a
  115 Ggt → Agt CpG Gly → Ser 1 5b 2 1 2a 1
  116 gGt → gAt   Gly → Asp 2 2a     1
  128 gGt → gAt   Gly → Asp 1 2     2
  176 tGt → tAt   Cys → Tyr       1
  262 Gat → Aat   Asp → Asn 1      
  274 Gat → Aat   Asp → Asn 1   1   1  
  281 gGt → gAt   Gly → Asp       1
  284 gGt → gAt   Gly → Asp       1
  367 Gat → Aat   Asp → Asn      1  
  401 tGg → tAg   Trp → Stop 2a 4a     1
  402 tgG → tgA   Trp → Stop     1   1
  406 Gaa → Aaa   Glu → Lys   3b     
  416 tGg → tAg   Trp → Stop   1     
  417 tgG → tgA   Trp → Stop 1 1 4b    
  418 Gat → Aat   Asp → Asn 2a 2a   1 2a 2a
A:T → G:C 25 Tgg → Cgg   Trp → Arg 1      
  41 aTc → aCc   Ile → Thr 1      
  56 cTc → cCc   Leu → Pro 1 8a 1    
  149 cTg → cCg   Leu → Pro   1     1
  415 Tgg → Cgg   Trp → Arg   1     
Transversion              
G:C → T:A 3 atG → atT   Met → Ile   1     
  7 Gaa → Taa CpG Glu → Stop   1     
  15 taC → taA   Tyr → Stop   1     
  26 tGg → tTg   Trp → Leu       1
  37 Cag → Aag   Gln → Lys    1    
  79 Gaa → Taa   Glu → Stop      1  
  92 gGc → gTc   Gly → Val      1  
  101 gCc → gAc   Ala → Asp      1  
  108 agC → agA   Ser → Arg     2   
  110 cGt → cTt CpG Arg → Leu       1
  115 Ggt → Tgt CpG Gly → Cys     2 1 2a
  116 gGt → gTt   Gly → Val   2a     1
  140 gCg → gAg CpG Ala → Glu 1    1 3a 1
  143 cGt → cTt CpG Arg → Leu     8a 1  
  145 Gaa → Taa   Glu → Stop 1 1 1    
  185 aGc → aTc   Ser → Ile 1      1
  186 agC → agA   Ser → Arg 1      
  189 taC → taA CpG Tyr → Stop    1 3a 1  
  208 Gag → Tag CpG Glu → Stop     3 1  
  244 Gaa → Taa CpG Glu → Stop 1 2a   2 1 1
  262 Gat → Tat   Asp → Tyr    1    
  281 gGt → gTt   Gly → Val      1  
  304 Gaa → Taa   Glu → Stop 1 1 1    
  320 gCg → gAg CpG Ala → Glu 1      1
  401 tGg → tTg   Trp → Leu   2a     
  402 tgG → tgT   Trp → Cys   1   1 4b  
  406 Gaa → Taa   Glu → Stop   2a 1   3b  
  409 Cag → Aag   Gln → Lys 1      
  413 cCg → cAg CpG Pro → Gln   1   1   
G:C → C:G 6 agC → agG CpG Ser → Arg   1     
  50 cGt → cCt CpG Arg → Pro     1   
  110 cGt → cCt CpG Arg → Pro      1  
  112 Ggc → Cgc   Gly → Arg 1     1  
  125 cCg → cGg CpG Pro → Arg   1   2a   
  127 Ggt → Cgt   Gly → Arg       1
  130 Gcg → Ccg   Ala → Pro      1 1
  139 Gcg → Ccg   Ala → Pro      1  
  186 agC → agG   Ser → Arg 2 1   1   
  206 cGc → cCc CpG Arg → Pro      1  
  280 Ggt → Cgt CpG Gly → Arg    1 1   
  289 Gcg → Ccg   Ala → Pro     1   
  290 gCg → gGg CpG Ala → Gly 1      
  340 Gca → Cca CpG Ala → Pro 1 1 1 1 4b 1
  413 cCg → cGg CpG Pro → Arg      1  
  418 Gat → Cat   Asp → His      1  
  442 Cca → Gca   Pro → Ala 1     1  
A:T → T:A 10 Aaa → Taa   Lys → Stop   1     
  25 Tgg → Agg   Trp → Arg      2a 1
  44 caT → caA   His → Gln      1  
  88 Aaa → Taa   Lys → Stop       2
  146 gAa → gTa   Glu → Val   3a     
  164 gTc → gAc   Val → Asp    2    
  187 Tac → Aac   Tyr → Asn 1      
  365 gTt → gAt   Val → Asp      1  
  375 taT → taA   Tyr → Stop   1     
  419 gAt → gTt   Asp → Val    2a    
  458 tAa → tTa   Stop → Leu   1     
  459 taA → taT   Stop → Tyr    1    
A:T → C:G 1 Atg → Ctg   Met → Leu    1    
  56 cTc → cGc   Leu → Arg    1    1
  106 Agc → Cgc   Ser → Arg   1 1    
  134 tTa → tGa   Leu → Stop   1     
  312 taT → taG   Tyr → Stop       1
  419 gAt → gCt   Asp → Ala   1     1
ᅟ
Deletion 8–12 gAAAAAt → gAAAAt 1 1 1    
−1 base 13 aTa → aa   1     
  26–28 tGGGa → tGGa      1 1
  34–35 gTTg → gTg 1      
  67 aCt → at      1  
  86–87 tGGa → tGa      1  
  101–102 gCCg → gCg      1  
  114 gCg → gg    1    
  124–125 aCCg → aCg       1
  126–128 cGGGt → cGGt   1    1  
  129 gTg → gg   1     
  170–171 aCCg → aCg    1   2a  
  176 tGt → tt       1
  198 aCa → aa     1   
  217 aGt → at      1  
  244 cGa → ca     1   
  247–248 aGGc → aGc       1
  249 gCt → gt    1    
- 266 gAc → gc    1    
  270–271 tGGt → tGt   2a   1   
  278–279 aCCg → aCg      1  
  285 gTa → ga      1  
  293–294 gTTg → gTg      1  
  315–318 cAAAAg → cAAAg      1  
  319 aGc → ac   1    1  
  332–333 aCCa → aCa   1     
  401–402 tGGa → tGa    1    
  416–418 tGGGa → tGGa 1 1    1  
  442–443 gCCa → gCa     1   
>2 107–109 aGCCg → ag      2  
  114–120 gCGGTCTGg → gg       1
  129–139 gTGCGTTACTGGc → gc    2    
  140–152 gCGCGTGAACTGGGt → gt   1     
  161–442      1     
  243–248 gCGAAGGc → gc       1
  300–306 tTCGTGAAa → ta       1
  375–380 aTGTTGTt → at    2    
Insertion 25 cTg → cTTg      1  
  75 ct → cAt   3a     
  124 ac → aTc      2a  
  136 aCt → aCCt      1  
  223–225 gAAAc → gAAAAc   1     
  229 cGc → cGGc       1
  286 gt → gATACCGGTGGt   1     
  335–337 aTCTt → aTCTTCTt       1
  362 cTg → cTTg      1  
  390 cc → cTc      1  
  392–393 cAAg → cAAAg       1
  401–402 tGGa → tGGGa    1 2   
Other 26–27 tGGg → tTg     1   
  59–60 gCAa → gGa      1  
  100–102 tGCCg → tTg      1  
  140–141 gCGc → gAAc     1   
  304 tGa → tAAa   1     
Total      38 92 43 42 79 49
  1. a, b, and cMutations found in 2, 3, and 4 different mice, respectively
  2. dCombined data of our previous studies [11, 17, 18]
  3. eOur previous data [11]
  4. CpG: mutation at CpG site
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Genes and Environment

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