Alterations in the mutagenicity and mutation spectrum induced by benzo[a]pyrene instilled in the lungs of gpt delta mice of various ages

Aoki, Yasunobu; Hashimoto, Akiko H.; Sugawara, Yoshiki; Hiyoshi-Arai, Kyoko; Goto, Sataro; Masumura, Kenichi; Nohmi, Takehiko

doi:10.1186/s41021-015-0004-x

Table 3 DNA sequence analysis of gpt mutations obtained from the lung of B[a]P-instilled and control mice

From: Alterations in the mutagenicity and mutation spectrum induced by benzo[a]pyrene instilled in the lungs of gpt delta mice of various ages

Type of mutation	Nucleo-tide #	Sequence		Change		Amino acid change			Number
									Control			B[a]P
									Months			Months
									3^d	11	24	3^e	11	24
Base substitution
Transition
G:C → A:T	26	tGg	→	tAg		Trp	→	Stop			1		2^a	1
	27	tgG	→	tgA		Trp	→	Stop			1		1
	37	Cag	→	Tag		Gln	→	Stop			1		2	1
	58	Gca	→	Aca	CpG	Ala	→	Thr			1
	64	Cga	→	Tga	CpG	Arg	→	Stop	1	5^b	1		5^b	1
	86	tGg	→	tAg		Trp	→	Stop		3^b		1
	87	tgG	→	tgA		Trp	→	Stop		2^a
	92	gGc	→	gAc		Gly	→	Asp		2^a
	110	cGt	→	cAt	CpG	Arg	→	His	5^c	4	3^b		4^b	6^a
	115	Ggt	→	Agt	CpG	Gly	→	Ser	1	5^b	2	1	2^a	1
	116	gGt	→	gAt		Gly	→	Asp	2	2^a				1
	128	gGt	→	gAt		Gly	→	Asp	1	2				2
	176	tGt	→	tAt		Cys	→	Tyr						1
	262	Gat	→	Aat		Asp	→	Asn	1
	274	Gat	→	Aat		Asp	→	Asn	1		1		1
	281	gGt	→	gAt		Gly	→	Asp						1
	284	gGt	→	gAt		Gly	→	Asp						1
	367	Gat	→	Aat		Asp	→	Asn					1
	401	tGg	→	tAg		Trp	→	Stop	2^a	4^a				1
	402	tgG	→	tgA		Trp	→	Stop				1		1
	406	Gaa	→	Aaa		Glu	→	Lys		3^b
	416	tGg	→	tAg		Trp	→	Stop		1
	417	tgG	→	tgA		Trp	→	Stop	1	1	4^b
	418	Gat	→	Aat		Asp	→	Asn	2^a	2^a		1	2^a	2^a
A:T → G:C	25	Tgg	→	Cgg		Trp	→	Arg	1
	41	aTc	→	aCc		Ile	→	Thr	1
	56	cTc	→	cCc		Leu	→	Pro	1	8^a	1
	149	cTg	→	cCg		Leu	→	Pro		1				1
	415	Tgg	→	Cgg		Trp	→	Arg		1
Transversion
G:C → T:A	3	atG	→	atT		Met	→	Ile		1
	7	Gaa	→	Taa	CpG	Glu	→	Stop		1
	15	taC	→	taA		Tyr	→	Stop		1
	26	tGg	→	tTg		Trp	→	Leu						1
	37	Cag	→	Aag		Gln	→	Lys			1
	79	Gaa	→	Taa		Glu	→	Stop					1
	92	gGc	→	gTc		Gly	→	Val					1
	101	gCc	→	gAc		Ala	→	Asp					1
	108	agC	→	agA		Ser	→	Arg				2
	110	cGt	→	cTt	CpG	Arg	→	Leu						1
	115	Ggt	→	Tgt	CpG	Gly	→	Cys				2	1	2^a
	116	gGt	→	gTt		Gly	→	Val		2^a				1
	140	gCg	→	gAg	CpG	Ala	→	Glu	1			1	3^a	1
	143	cGt	→	cTt	CpG	Arg	→	Leu				8^a	1
	145	Gaa	→	Taa		Glu	→	Stop	1	1	1
	185	aGc	→	aTc		Ser	→	Ile	1					1
	186	agC	→	agA		Ser	→	Arg	1
	189	taC	→	taA	CpG	Tyr	→	Stop			1	3^a	1
	208	Gag	→	Tag	CpG	Glu	→	Stop				3	1
	244	Gaa	→	Taa	CpG	Glu	→	Stop	1	2^a		2	1	1
	262	Gat	→	Tat		Asp	→	Tyr			1
	281	gGt	→	gTt		Gly	→	Val					1
	304	Gaa	→	Taa		Glu	→	Stop	1	1	1
	320	gCg	→	gAg	CpG	Ala	→	Glu	1					1
	401	tGg	→	tTg		Trp	→	Leu		2^a
	402	tgG	→	tgT		Trp	→	Cys		1		1	4^b
	406	Gaa	→	Taa		Glu	→	Stop		2^a	1		3^b
	409	Cag	→	Aag		Gln	→	Lys	1
	413	cCg	→	cAg	CpG	Pro	→	Gln		1		1
G:C → C:G	6	agC	→	agG	CpG	Ser	→	Arg		1
	50	cGt	→	cCt	CpG	Arg	→	Pro				1
	110	cGt	→	cCt	CpG	Arg	→	Pro					1
	112	Ggc	→	Cgc		Gly	→	Arg	1				1
	125	cCg	→	cGg	CpG	Pro	→	Arg		1		2^a
	127	Ggt	→	Cgt		Gly	→	Arg						1
	130	Gcg	→	Ccg		Ala	→	Pro					1	1
	139	Gcg	→	Ccg		Ala	→	Pro					1
	186	agC	→	agG		Ser	→	Arg	2	1		1
	206	cGc	→	cCc	CpG	Arg	→	Pro					1
	280	Ggt	→	Cgt	CpG	Gly	→	Arg			1	1
	289	Gcg	→	Ccg		Ala	→	Pro				1
	290	gCg	→	gGg	CpG	Ala	→	Gly	1
	340	Gca	→	Cca	CpG	Ala	→	Pro	1	1	1	1	4^b	1
	413	cCg	→	cGg	CpG	Pro	→	Arg					1
	418	Gat	→	Cat		Asp	→	His					1
	442	Cca	→	Gca		Pro	→	Ala	1				1
A:T → T:A	10	Aaa	→	Taa		Lys	→	Stop		1
	25	Tgg	→	Agg		Trp	→	Arg					2^a	1
	44	caT	→	caA		His	→	Gln					1
	88	Aaa	→	Taa		Lys	→	Stop						2
	146	gAa	→	gTa		Glu	→	Val		3^a
	164	gTc	→	gAc		Val	→	Asp			2
	187	Tac	→	Aac		Tyr	→	Asn	1
	365	gTt	→	gAt		Val	→	Asp					1
	375	taT	→	taA		Tyr	→	Stop		1
	419	gAt	→	gTt		Asp	→	Val			2^a
	458	tAa	→	tTa		Stop	→	Leu		1
	459	taA	→	taT		Stop	→	Tyr			1
A:T → C:G	1	Atg	→	Ctg		Met	→	Leu			1
	56	cTc	→	cGc		Leu	→	Arg			1			1
	106	Agc	→	Cgc		Ser	→	Arg		1	1
	134	tTa	→	tGa		Leu	→	Stop		1
	312	taT	→	taG		Tyr	→	Stop						1
	419	gAt	→	gCt		Asp	→	Ala		1				1
ᅟ
Deletion	8–12	gAAAAAt					→	gAAAAt	1	1	1
−1 base	13	aTa					→	aa		1
	26–28	tGGGa					→	tGGa					1	1
	34–35	gTTg					→	gTg	1
	67	aCt					→	at					1
	86–87	tGGa					→	tGa					1
	101–102	gCCg					→	gCg					1
	114	gCg					→	gg			1
	124–125	aCCg					→	aCg						1
	126–128	cGGGt					→	cGGt		1			1
	129	gTg					→	gg		1
	170–171	aCCg					→	aCg			1		2^a
	176	tGt					→	tt						1
	198	aCa					→	aa				1
	217	aGt					→	at					1
	244	cGa					→	ca				1
	247–248	aGGc					→	aGc						1
	249	gCt					→	gt			1
-	266	gAc					→	gc			1
	270–271	tGGt					→	tGt		2^a		1
	278–279	aCCg					→	aCg					1
	285	gTa					→	ga					1
	293–294	gTTg					→	gTg					1
	315–318	cAAAAg					→	cAAAg					1
	319	aGc					→	ac		1			1
	332–333	aCCa					→	aCa		1
	401–402	tGGa					→	tGa			1
	416–418	tGGGa					→	tGGa	1	1			1
	442–443	gCCa					→	gCa				1
>2	107–109	aGCCg					→	ag					2
	114–120	gCGGTCTGg					→	gg						1
	129–139	gTGCGTTACTGGc					→	gc			2
	140–152	gCGCGTGAACTGGGt					→	gt		1
	161–442									1
	243–248	gCGAAGGc					→	gc						1
	300–306	tTCGTGAAa					→	ta						1
	375–380	aTGTTGTt					→	at			2
Insertion	25	cTg					→	cTTg					1
	75	ct					→	cAt		3^a
	124	ac					→	aTc					2^a
	136	aCt					→	aCCt					1
	223–225	gAAAc					→	gAAAAc		1
	229	cGc					→	cGGc						1
	286	gt					→	gATACCGGTGGt		1
	335–337	aTCTt					→	aTCTTCTt						1
	362	cTg					→	cTTg					1
	390	cc					→	cTc					1
	392–393	cAAg					→	cAAAg						1
	401–402	tGGa					→	tGGGa			1	2
Other	26–27	tGGg					→	tTg				1
	59–60	gCAa					→	gGa					1
	100–102	tGCCg					→	tTg					1
	140–141	gCGc					→	gAAc				1
	304	tGa					→	tAAa		1
Total									38	92	43	42	79	49

^{a, b, and c}Mutations found in 2, 3, and 4 different mice, respectively
^dCombined data of our previous studies [11, 17, 18]
^eOur previous data [11]
CpG: mutation at CpG site

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