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Table 3 DNA sequence analysis of gpt mutations obtained from the lung of B[a]P-instilled and control mice

From: Alterations in the mutagenicity and mutation spectrum induced by benzo[a]pyrene instilled in the lungs of gpt delta mice of various ages

Type of mutation Nucleo-tide # Sequence Change Amino acid change Number
Control B[a]P
Months Months
3d 11 24 3e 11 24
Base substitution             
Transition             
G:C → A:T 26 tGg tAg   Trp Stop    1   2a 1
  27 tgG tgA   Trp Stop    1   1  
  37 Cag Tag   Gln Stop    1   2 1
  58 Gca Aca CpG Ala Thr    1    
  64 Cga Tga CpG Arg Stop 1 5b 1   5b 1
  86 tGg tAg   Trp Stop   3b   1   
  87 tgG tgA   Trp Stop   2a     
  92 gGc gAc   Gly Asp   2a     
  110 cGt cAt CpG Arg His 5c 4 3b   4b 6a
  115 Ggt Agt CpG Gly Ser 1 5b 2 1 2a 1
  116 gGt gAt   Gly Asp 2 2a     1
  128 gGt gAt   Gly Asp 1 2     2
  176 tGt tAt   Cys Tyr       1
  262 Gat Aat   Asp Asn 1      
  274 Gat Aat   Asp Asn 1   1   1  
  281 gGt gAt   Gly Asp       1
  284 gGt gAt   Gly Asp       1
  367 Gat Aat   Asp Asn      1  
  401 tGg tAg   Trp Stop 2a 4a     1
  402 tgG tgA   Trp Stop     1   1
  406 Gaa Aaa   Glu Lys   3b     
  416 tGg tAg   Trp Stop   1     
  417 tgG tgA   Trp Stop 1 1 4b    
  418 Gat Aat   Asp Asn 2a 2a   1 2a 2a
A:T → G:C 25 Tgg Cgg   Trp Arg 1      
  41 aTc aCc   Ile Thr 1      
  56 cTc cCc   Leu Pro 1 8a 1    
  149 cTg cCg   Leu Pro   1     1
  415 Tgg Cgg   Trp Arg   1     
Transversion              
G:C → T:A 3 atG atT   Met Ile   1     
  7 Gaa Taa CpG Glu Stop   1     
  15 taC taA   Tyr Stop   1     
  26 tGg tTg   Trp Leu       1
  37 Cag Aag   Gln Lys    1    
  79 Gaa Taa   Glu Stop      1  
  92 gGc gTc   Gly Val      1  
  101 gCc gAc   Ala Asp      1  
  108 agC agA   Ser Arg     2   
  110 cGt cTt CpG Arg Leu       1
  115 Ggt Tgt CpG Gly Cys     2 1 2a
  116 gGt gTt   Gly Val   2a     1
  140 gCg gAg CpG Ala Glu 1    1 3a 1
  143 cGt cTt CpG Arg Leu     8a 1  
  145 Gaa Taa   Glu Stop 1 1 1    
  185 aGc aTc   Ser Ile 1      1
  186 agC agA   Ser Arg 1      
  189 taC taA CpG Tyr Stop    1 3a 1  
  208 Gag Tag CpG Glu Stop     3 1  
  244 Gaa Taa CpG Glu Stop 1 2a   2 1 1
  262 Gat Tat   Asp Tyr    1    
  281 gGt gTt   Gly Val      1  
  304 Gaa Taa   Glu Stop 1 1 1    
  320 gCg gAg CpG Ala Glu 1      1
  401 tGg tTg   Trp Leu   2a     
  402 tgG tgT   Trp Cys   1   1 4b  
  406 Gaa Taa   Glu Stop   2a 1   3b  
  409 Cag Aag   Gln Lys 1      
  413 cCg cAg CpG Pro Gln   1   1   
G:C → C:G 6 agC agG CpG Ser Arg   1     
  50 cGt cCt CpG Arg Pro     1   
  110 cGt cCt CpG Arg Pro      1  
  112 Ggc Cgc   Gly Arg 1     1  
  125 cCg cGg CpG Pro Arg   1   2a   
  127 Ggt Cgt   Gly Arg       1
  130 Gcg Ccg   Ala Pro      1 1
  139 Gcg Ccg   Ala Pro      1  
  186 agC agG   Ser Arg 2 1   1   
  206 cGc cCc CpG Arg Pro      1  
  280 Ggt Cgt CpG Gly Arg    1 1   
  289 Gcg Ccg   Ala Pro     1   
  290 gCg gGg CpG Ala Gly 1      
  340 Gca Cca CpG Ala Pro 1 1 1 1 4b 1
  413 cCg cGg CpG Pro Arg      1  
  418 Gat Cat   Asp His      1  
  442 Cca Gca   Pro Ala 1     1  
A:T → T:A 10 Aaa Taa   Lys Stop   1     
  25 Tgg Agg   Trp Arg      2a 1
  44 caT caA   His Gln      1  
  88 Aaa Taa   Lys Stop       2
  146 gAa gTa   Glu Val   3a     
  164 gTc gAc   Val Asp    2    
  187 Tac Aac   Tyr Asn 1      
  365 gTt gAt   Val Asp      1  
  375 taT taA   Tyr Stop   1     
  419 gAt gTt   Asp Val    2a    
  458 tAa tTa   Stop Leu   1     
  459 taA taT   Stop Tyr    1    
A:T → C:G 1 Atg Ctg   Met Leu    1    
  56 cTc cGc   Leu Arg    1    1
  106 Agc Cgc   Ser Arg   1 1    
  134 tTa tGa   Leu Stop   1     
  312 taT taG   Tyr Stop       1
  419 gAt gCt   Asp Ala   1     1
Deletion 8–12 gAAAAAt gAAAAt 1 1 1    
−1 base 13 aTa aa   1     
  26–28 tGGGa tGGa      1 1
  34–35 gTTg gTg 1      
  67 aCt at      1  
  86–87 tGGa tGa      1  
  101–102 gCCg gCg      1  
  114 gCg gg    1    
  124–125 aCCg aCg       1
  126–128 cGGGt cGGt   1    1  
  129 gTg gg   1     
  170–171 aCCg aCg    1   2a  
  176 tGt tt       1
  198 aCa aa     1   
  217 aGt at      1  
  244 cGa ca     1   
  247–248 aGGc aGc       1
  249 gCt gt    1    
- 266 gAc gc    1    
  270–271 tGGt tGt   2a   1   
  278–279 aCCg aCg      1  
  285 gTa ga      1  
  293–294 gTTg gTg      1  
  315–318 cAAAAg cAAAg      1  
  319 aGc ac   1    1  
  332–333 aCCa aCa   1     
  401–402 tGGa tGa    1    
  416–418 tGGGa tGGa 1 1    1  
  442–443 gCCa gCa     1   
>2 107–109 aGCCg ag      2  
  114–120 gCGGTCTGg gg       1
  129–139 gTGCGTTACTGGc gc    2    
  140–152 gCGCGTGAACTGGGt gt   1     
  161–442      1     
  243–248 gCGAAGGc gc       1
  300–306 tTCGTGAAa ta       1
  375–380 aTGTTGTt at    2    
Insertion 25 cTg cTTg      1  
  75 ct cAt   3a     
  124 ac aTc      2a  
  136 aCt aCCt      1  
  223–225 gAAAc gAAAAc   1     
  229 cGc cGGc       1
  286 gt gATACCGGTGGt   1     
  335–337 aTCTt aTCTTCTt       1
  362 cTg cTTg      1  
  390 cc cTc      1  
  392–393 cAAg cAAAg       1
  401–402 tGGa tGGGa    1 2   
Other 26–27 tGGg tTg     1   
  59–60 gCAa gGa      1  
  100–102 tGCCg tTg      1  
  140–141 gCGc gAAc     1   
  304 tGa tAAa   1     
Total      38 92 43 42 79 49
  1. a, b, and cMutations found in 2, 3, and 4 different mice, respectively
  2. dCombined data of our previous studies [11, 17, 18]
  3. eOur previous data [11]
  4. CpG: mutation at CpG site