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Fig. 8 | Genes and Environment

Fig. 8

From: An in vitro method for detecting genetic toxicity based on inhibition of RNA synthesis by DNA lesions

Fig. 8

Analysis of PCR products generated by RT-PCR from RNA transcripts of cisplatin-treated DNA templates. a Scheme for detecting transcription inhibition using RNA transcripts from cisplatin-treated DNA templates (drug/nucleotide ratio = 0.005). b Agarose gel (1 %) demonstrating RNA transcripts from cisplatin-treated DNA templates. M: size marker. Lane 1: RNA transcripts from mock DNA templates. Lane 2: RNA transcripts from cisplatin-treated DNA templates. c Agarose gel (1 %) demonstrating RT-PCR products (301 bp) from cisplatin-treated DNA templates. M: size marker. Odd lanes: RT-PCR products from mock DNA templates. Even lanes: RT-PCR products from cisplatin-treated DNA templates. DNA was amplified for 9 (lanes 1 and 2), 12 (lanes 3 and 4), 15 (lanes 5 and 6), 18 (lanes 7 and 8), or 21 (lanes 9 and 10) PCR cycles. Lanes 11 and 12: no RT reaction

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Genes and Environment

ISSN: 1880-7062

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