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Table 2 Spectra of mutations detected in the supF gene

From: Induction of action-at-a-distance mutagenesis by 8-oxo-7,8-dihydroguanine in DNA pol λ-knockdown cells

 

G : C

GO : C

 

Control

si-pol λ

Control

si-pol λ

mutations at position 122

    

 G : C - > A : T

0 (0)

0 (0)

0 (0)

3 (4)

 G : C - > T : A

0 (0)

0 (0)

38 (56)

31 (42)

 G : C - > C : G

0 (0)

0 (0)

0 (0)

2 (3)

mutations at other positions

    

 transition

    

  A : T - > G : C

3 (15)

0 (0)

0 (0)

0 (0)

  G : C - > A : T

4 (20)

7 (37)

9 (13)

23 (31)

 transversion

    

  A : T - > T : A

0 (0)

0 (0)

0 (0)

0 (0)

  A : T - > C : G

0 (0)

1 (5)

4 (6)

0 (0)

  G : C - > T : A

2 (10)

3 (16)

10 (15)

5 (7)

  G : C - > C : G

2 (10)

2 (11)

11 (16)

17 (23)

 small insertion (1–2 bp)

0 (0)

0 (0)

0 (0)

0 (0)

 large insertion (>2 bp)

0 (0)

1 (5)

0 (0)

0 (0)

 small deletion (1–2 bp)

0 (0)

1 (5)

0 (0)

0 (0)

 large deletion (>2 bp)

9 (45)

4 (21)

4 (6)

5 (7)

 others

0 (0)

0 (0)

0 (0)

0 (0)

total mutations

20

19

76

86

total colonies analyzed

20 (100)

19 (100)

68 (100)

74 (100)

  1. All data are represented as cases found (%)