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Fig. 2 | Genes and Environment

Fig. 2

From: Mutation assay using single-molecule real-time (SMRTTM) sequencing technology

Fig. 2

Detection of mutations, DNA damage and mismatches by mapping of raw reads of the SMRT sequencer. a Example of ENU induction of an alkylated base (O6-ethyl-guanine) in genomic DNA, which will induce a G to A mutation after the 2nd round of replication. b Examples of mapped reads. In cases of a real mutation, the same base is clearly called in both the forward and reverse reads. In cases of DNA damage, one strand is mapped clearly but the other strand is not. In cases of mismatch, both the forward and reverse reads are mapped clearly but different bases are called between the forward and reverse reads

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