Mutation assay using single-molecule real-time (SMRTTM) sequencing technology

Matsuda, Tomonari; Matsuda, Shun; Yamada, Masami

doi:10.1186/s41021-015-0017-5

Genes and Environment

Table 3 Details of the 160 base substitutions called at the first screening in the ENU-treated sample

From: Mutation assay using single-molecule real-time (SMRT^TM) sequencing technology

Reference position	Reference position	Forward read				Reverse read				Comment	Judgement	p-value^**
Reference position	Reference position	Most dominant allele	Coverage	Read count	p-value^*	Most dominant allele	Coverage	Read count	p-value^*	Comment	Judgement	p-value^**
146824	G	A	22	19	1.2E-20	A	22	18	9.9E-19		Mutation	0
994061	G	A	20	19	2.9E-22	A	20	17	2.4E-18		Mutation	0
2007634	G	A	33	24	5.9E-22	A	31	25	2.9E-25		Mutation	0
2044677	C	T	24	19	3.9E-19	T	21	18	1.7E-19		Mutation	0
2724713	C	T	32	21	3.4E-17	T	33	22	2.5E-18		Mutation	0
2747120	G	A	34	24	2.9E-21	A	37	31	4.0E-32		Mutation	0
2871399	G	A	32	26	2.1E-26	A	34	28	1.1E-28		Mutation	0
2930794	G	A	26	23	2.8E-25	A	24	18	3.0E-17		Mutation	0
3007696	A	G	45	33	4.8E-30	G	47	36	4.0E-34		Mutation	0
3322100	C	T	21	18	1.7E-19	T	22	19	1.2E-20		Mutation	0
3666060	G	A	29	23	5.7E-23	A	30	22	2.2E-20		Mutation	0
3695370	G	A	20	17	2.4E-18	A	21	18	1.7E-19		Mutation	0
3708252	A	G	29	26	9.5E-29	G	31	25	2.9E-25		Mutation	0
3863986	G	A	18	16	5.8E-18	A	19	16	3.5E-17		Mutation	0
3961843	G	A	25	20	2.8E-20	A	25	23	4.3E-26		Mutation	0
4320817	C	T	21	18	1.7E-19	T	21	18	1.7E-19		Mutation	0
2171812	G	A	16	15	1.3E-17	A	17	15	8.4E-17		Mutation	1.1E-16
327560	C	T	23	17	4.3E-16	T	24	20	4.9E-21		Mutation	4.4E-16
2209612	A	G	16	14	1.2E-15	G	15	14	2.0E-16		Mutation	1.4E-15
2705366	G	A	24	17	2.2E-15	A	24	22	6.3E-25		Mutation	2.2E-15
2215678	C	T	30	19	6.1E-15	T	30	26	6.0E-28		Mutation	6.1E-15
3881583	C	T	15	13	1.8E-14	T	14	14	3.1E-17		Mutation	1.8E-14
1368298	G	A	16	13	1.1E-13	A	17	17	9.5E-21		Mutation	1.1E-13
4840145	G	A	16	13	1.1E-13	A	18	14	4.2E-14		Mutation	1.5E-13
390064	C	T	17	13	6.1E-13	T	19	16	3.5E-17		Mutation	6.1E-13
733247	C	T	17	13	6.1E-13	T	18	16	5.8E-18		Mutation	6.1E-13
3257503	G	A	17	13	6.1E-13	A	17	16	9.1E-19		Mutation	6.1E-13
935658	G	A	18	15	5.0E-16	A	17	13	6.1E-13		Mutation	6.1E-13
2316694	C	T	17	14	7.4E-15	T	17	13	6.1E-13		Mutation	6.2E-13
414142	G	A	12	11	6.7E-13	A	12	12	6.9E-15		Mutation	6.7E-13
556175	G	A	13	12	4.4E-14	A	12	11	6.7E-13		Mutation	7.1E-13
355651	C	T	38	30	1.8E-29	T	36	20	7.3E-13		Mutation	7.3E-13
748721	C	T	14	12	2.7E-13	T	12	11	6.7E-13		Mutation	9.4E-13
2715604	C	T	20	14	1.2E-12	T	24	20	4.9E-21		Mutation	1.2E-12
2504585	C	T	10	10	1.6E-12	T	10	10	1.6E-12		Mutation	3.2E-12
688445	G	A	12	11	6.7E-13	A	11	10	1.0E-11		Mutation	1.1E-11
222807	C	T	18	16	5.8E-18	T	19	13	1.7E-11		Mutation	1.7E-11
4652102	G	A	19	13	1.7E-11	A	22	20	1.3E-22		Mutation	1.7E-11
3117258	G	A	25	19	2.2E-18	A	22	14	3.0E-11		Mutation	3.0E-11
1005055	C	T	16	12	8.9E-12	T	14	11	2.4E-11		Mutation	3.2E-11
2264426	G	A	11	10	1.0E-11	A	9	9	2.5E-11		Mutation	3.5E-11
992465	C	T	14	11	2.4E-11	T	14	11	2.4E-11		Mutation	4.7E-11
1076365	G	T	12	10	6.1E-11	T	12	11	6.7E-13		Mutation	6.2E-11
458994	C	A	29	26	9.5E-29	A	23	14	1.4E-10		Mutation	1.4E-10
3062433	C	T	18	14	4.2E-14	T	13	10	3.5E-10		Mutation	3.5E-10
421079	G	T	8	8	4.0E-10	T	9	9	2.5E-11		Mutation	4.2E-10
4736812	C	A	24	14	6.4E-10	A	18	16	5.8E-18		Mutation	6.4E-10
2957288	C	T	12	12	6.9E-15	T	11	9	9.4E-10		Mutation	9.4E-10
3872165	C	T	11	9	9.4E-10	T	10	10	1.6E-12		Mutation	9.4E-10
278409	G	T	19	12	1.2E-09	T	23	14	1.4E-10		Mutation	1.4E-09
2861538	G	A	15	13	1.8E-14	A	14	10	1.9E-09		Mutation	1.9E-09
1408682	G	A	9	8	2.4E-09	A	9	9	2.5E-11		Mutation	2.5E-09l
272653	C	A	10	9	1.6E-10	A	9	8	2.4E-09		Mutation	2.6E-09
2757635	G	T	9	8	2.4E-09	T	8	8	4.0E-10		Mutation	2.8E-09
4148066	C	A	9	8	2.4E-09	A	8	8	4.0E-10		Mutation	2.8E-09
206275		T	17	11	3.6E-09	T	14	11	2.4E-11		Mutation	3.6E-09
250264	C	T	9	8	2.4E-09	T	9	8	2.4E-09		Mutation	4.9E-09
2425294	C	T	9	8	2.4E-09	T	9	8	2.4E-09		Mutation	4.9E-09
4431921	G	A	12	9	5.4E-09	A	12	10	6.1E-11		Mutation	5.4E-09
909863	C	T	31	16	5.5E-09	T	31	17	9.6E-11		Mutation	5.6E-09
1085221	G	A	12	9	5.4E-09	A	12	9	5.4E-09		Mutation	1.1E-08
2250730	G	A	10	10	1.6E-12	A	10	8	1.5E-08		Mutation	1.5E-08
662822	G	A	21	12	2.8E-08	A	19	18	4.3E-21		Mutation	2.8E-08
731542	C	T	13	9	2.9E-08	T	13	11	4.1E-12		Mutation	2.9E-08
412934	G	A	8	7	3.9E-08	A	10	8	1.5E-08		Mutation	5.3E-08
2104411	C	T	11	8	8.2E-08	T	12	10	6.1E-11		Mutation	8.2E-08
4189314	G	A	15	13	1.8E-14	A	14	9	1.5E-07		Mutation	1.5E-07
3364045	C	T	14	9	1.5E-07	T	15	12	1.6E-12		Mutation	1.5E-07
2795479	C	T	6	6	1.0E-07	T	6	6	1.0E-07		Mutation	2.1E-07
555449	G	A	9	7	2.3E-07	A	9	9	2.5E-11		Mutation	2.3E-07
1306236	G	A	12	8	4.5E-07	A	12	10	6.1E-11		Mutation	4.5E-07
4173104	G	A	9	7	2.3E-07	A	9	7	2.3E-07		Mutation	4.6E-07
2260312	C	T	8	7	3.9E-08	T	7	6	6.3E-07		Mutation	6.7E-07
2873628	G	A	6	6	1.0E-07	A	7	6	6.3E-07		Mutation	7.4E-07
1219556	C	T	9	7	2.3E-07	T	7	6	6.3E-07		Mutation	8.6E-07
3929806	C	T	17	13	6.1E-13	T	18	10	1.2E-06		Mutation	1.2E-06
719703	G	A	10	7	1.3E-06	A	9	8	2.4E-09		Mutation	1.3E-06
767167	C	T	10	7	1.3E-06	T	11	8	8.2E-08		Mutation	1.4E-06
4671425	C	T	5	5	1.7E-06	T	7	7	6.3E-09		Mutation	1.7E-06
74626	G	A	5	5	1.7E-06	A	6	6	1.0E-07		Mutation	1.8E-06
1556611	G	A	5	5	1.7E-06	A	6	6	1.0E-07		Mutation	1.8E-06
3771665	G	A	13	8	2.3E-06	A	13	10	3.5E-10		Mutation	2.3E-06
1277370	C	T	10	7	1.3E-06	T	10	7	1.3E-06		Mutation	2.6E-06
2831234	G	A	8	6	3.7E-06	A	8	8	4.0E-10		Mutation	3.7E-06
4834248	G	A	16	9	3.8E-06	A	17	15	8.4E-17		Mutation	3.8E-06
4640576	G	A	12	10	6.1E-11	A	11	7	6.9E-06		Mutation	6.9E-06
314407	C	T	11	7	6.9E-06	T	10	9	1.6E-10		Mutation	6.9E-06
1799318	G	A	9	8	2.4E-09	A	6	5	1.1E-05		Mutation	1.1E-05
2647267	T	C	6	5	1.1E-05	C	7	7	6.3E-09		Mutation	1.1E-05
1579929	G	A	6	6	1.0E-07	A	6	5	1.1E-05		Mutation	1.1E-05
2458998	C	T	6	6	1.0E-07	T	6	5	1.1E-05		Mutation	1.1E-05
3936247	C	T	14	13	3.0E-15	T	14	8	1.2E-05		Mutation	1.2E-05
4121383	G	A	14	8	1.2E-05	A	14	9	1.5E-07		Mutation	1.2E-05
1511517	G	A	5	5	1.7E-06	A	6	5	1.1E-05		Mutation	1.2E-05
2963125	G	A	5	5	1.7E-06	A	6	5	1.1E-05		Mutation	1.2E-05
2953567	G	A	9	8	2.4E-09	A	9	6	2.1E-05		Mutation	2.1E-05
4521210	G	A	9	6	2.1E-05	A	10	7	1.3E-06		Mutation	2.2E-05
1066165	C	T	4	4	3.0E-05	T	5	5	1.7E-06		Mutation	3.2E-05
4377924	G	A	5	5	1.7E-06	A	4	4	3.0E-05		Mutation	3.2E-05
655040	C	T	12	7	3.6E-05	T	12	11	6.7E-13		Mutation	3.6E-05
3801057	G	A	12	7	3.6E-05	A	12	8	4.5E-07		Mutation	3.6E-05
1064555	G	A	4	4	3.0E-05	A	6	5	1.1E-05		Mutation	4.1E-05
3156134	C	T	15	8	5.6E-05	T	12	8	4.5E-07		Mutation	5.7E-05
1090650	C	T	7	5	6.1E-05	T	8	8	4.0E-10		Mutation	6.1E-05
4836541	T	C	7	5	6.1E-05	C	8	8	4.0E-10		Mutation	6.1E-05
3417592	G	A	7	5	6.1E-05	A	8	7	3.9E-08		Mutation	6.1E-05
3188210	G	A	6	6	1.0E-07	A	7	5	6.1E-05		Mutation	6.1E-05
700494	C	T	7	5	6.1E-05	T	7	6	6.3E-07		Mutation	6.2E-05
3298937	C	T	7	6	6.3E-07	T	7	5	6.1E-05		Mutation	6.2E-05
496768	A	G	7	5	6.1E-05	G	5	5	1.7E-06		Mutation	6.3E-05
630974	G	A	7	5	6.1E-05	A	7	5	6.1E-05		Mutation	1.2E-04
4169252	G	A	5	4	1.8E-04	A	6	6	1.0E-07		Mutation	1.8E-04
2123568	G	A	5	4	1.8E-04	A	6	5	1.1E-05		Mutation	1.9E-04
3795698	G	A	6	5	1.1E-05	A	5	4	1.8E-04		Mutation	1.9E-04
1779923	G	A	7	5	6.1E-05	A	5	4	1.8E-04		Mutation	2.4E-04
3668382	G	A	19	9	3.1E-04	A	20	16	2.0E-16		Mutation	3.1E-04
2989782	G	A	8	5	3.4E-04	A	8	8	4.0E-10		Mutation	3.4E-04
1297655	C	T	8	5	3.4E-04	T	8	7	3.9E-08		Mutation	3.4E-04
3625847	A	G	8	5	3.4E-04	G	10	7	1.3E-06		Mutation	3.4E-04
4660505	G	A	17	8	9.7E-04	A	20	13	8.6E-11		Mutation	9.7E-04
4586383	C	T	6	4	1.0E-03	T	6	6	1.0E-07		Mutation	1.0E-03
2325510	C	T	5	4	1.8E-04	T	4	3	3.2E-03		Mutation	3.4E-03
4111137	G	A	5	4	1.8E-04	A	4	3	3.2E-03		Mutation	3.4E-03
2901163	C	T	8	5	3.4E-04	T	7	4	5.7E-03		Mutation	6.0E-03
3551802	G	A	7	4	5.7E-03	A	8	5	3.4E-04		Mutation	6.0E-03
4469079	C	T	10	8	1.5E-08	T	10	5	9.1E-03		Mutation	9.1E-03
4539546	G	A	5	3	0.02	A	6	4	1.0E-03		Mutation	0.02
4539738	G	A	5	5	1.7E-06	A	3	2	0.06		Mutation	0.06
2955452	G	A	6	3	0.10	A	7	7	6.3E-09		Mutation	0.10
4153066	G	A	8	6	3.7E-06	A	6	3	0.10		Mutation	0.10
4767697	C	T	6	5	1.1E-05	T	6	3	0.10		Mutation	0.10
4128014	C	T	6	3	0.10	T	6	4	1.0E-03		Mutation	0.10
2410269	G	A	7	3	0.23	A	8	5	3.4E-04		Mutation	0.23
3010834	C	C	18	16	5.8E-18	T	15	15	2.1E-18		Mismatch	0
3615885	C	C	21	21	2.1E-25	T	21	17	1.4E-17		Mismatch	0
4623405	A	T	71	63	3.4E-67	A	71	66	3.6E-73		Mismatch	0
2499952	G	A	15	14	2.0E-16	G	15	15	2.1E-18		Mismatch	2.2E-16
4452587	G	A	10	10	1.6E-12	G	10	9	1.6E-10		Mismatch	1.6E-10
3911612	C	C	21	13	4.2E-10	T	20	15	1.6E-14		Mismatch	4.2E-10
2608981	C	C	7	6	6.3E-07	T	8	8	4.0E-10		Mismatch	6.3E-07
128045	G	C	14	12	2.7E-13	G	12	7	3.6E-05		Mismatch	3.6E-05
3890711	G	A	7	4	5.7E-03	G	7	4	5.7E-03		Mismatch	0.01
2691271	C	T	7	7	6.3E-09	C or -	8	2			Damage
2750772	G	-	4	3	3.2E-03	A	5	5	1.7E-06		Damage
3648312	A	A or -	6	2		G	5	4	1.8E-04		Damage
4329658	C	T	5	4	1.8E-04	T,G,C,-	4	1			Damage
1412330	G									original allele	No
2298627	A									original allele	No
2846790	G									original allele	No
3386508	G									original allele	No
3386511	T									original allele	No
171648	C									No mutation	No
357352	A									No mutation	No
992221	G									No mutation	No
4831490	C									No mutation	No
1291152	G									edge of map	No
3591684	C									edge of map	No
4010951	G									edge of map	No
4065759	G									edge of map	No
4314300	G									edge of map	No

^*Probability that the real allele is not the most dominant allele
^**Probability that the Judgement is not correct

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ISSN: 1880-7062

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