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Table 3 Details of the 160 base substitutions called at the first screening in the ENU-treated sample

From: Mutation assay using single-molecule real-time (SMRTTM) sequencing technology

Reference position Reference position Forward read Reverse read Comment Judgement p-value**
Most dominant allele Coverage Read count p-value* Most dominant allele Coverage Read count p-value*
146824 G A 22 19 1.2E-20 A 22 18 9.9E-19   Mutation 0
994061 G A 20 19 2.9E-22 A 20 17 2.4E-18   Mutation 0
2007634 G A 33 24 5.9E-22 A 31 25 2.9E-25   Mutation 0
2044677 C T 24 19 3.9E-19 T 21 18 1.7E-19   Mutation 0
2724713 C T 32 21 3.4E-17 T 33 22 2.5E-18   Mutation 0
2747120 G A 34 24 2.9E-21 A 37 31 4.0E-32   Mutation 0
2871399 G A 32 26 2.1E-26 A 34 28 1.1E-28   Mutation 0
2930794 G A 26 23 2.8E-25 A 24 18 3.0E-17   Mutation 0
3007696 A G 45 33 4.8E-30 G 47 36 4.0E-34   Mutation 0
3322100 C T 21 18 1.7E-19 T 22 19 1.2E-20   Mutation 0
3666060 G A 29 23 5.7E-23 A 30 22 2.2E-20   Mutation 0
3695370 G A 20 17 2.4E-18 A 21 18 1.7E-19   Mutation 0
3708252 A G 29 26 9.5E-29 G 31 25 2.9E-25   Mutation 0
3863986 G A 18 16 5.8E-18 A 19 16 3.5E-17   Mutation 0
3961843 G A 25 20 2.8E-20 A 25 23 4.3E-26   Mutation 0
4320817 C T 21 18 1.7E-19 T 21 18 1.7E-19   Mutation 0
2171812 G A 16 15 1.3E-17 A 17 15 8.4E-17   Mutation 1.1E-16
327560 C T 23 17 4.3E-16 T 24 20 4.9E-21   Mutation 4.4E-16
2209612 A G 16 14 1.2E-15 G 15 14 2.0E-16   Mutation 1.4E-15
2705366 G A 24 17 2.2E-15 A 24 22 6.3E-25   Mutation 2.2E-15
2215678 C T 30 19 6.1E-15 T 30 26 6.0E-28   Mutation 6.1E-15
3881583 C T 15 13 1.8E-14 T 14 14 3.1E-17   Mutation 1.8E-14
1368298 G A 16 13 1.1E-13 A 17 17 9.5E-21   Mutation 1.1E-13
4840145 G A 16 13 1.1E-13 A 18 14 4.2E-14   Mutation 1.5E-13
390064 C T 17 13 6.1E-13 T 19 16 3.5E-17   Mutation 6.1E-13
733247 C T 17 13 6.1E-13 T 18 16 5.8E-18   Mutation 6.1E-13
3257503 G A 17 13 6.1E-13 A 17 16 9.1E-19   Mutation 6.1E-13
935658 G A 18 15 5.0E-16 A 17 13 6.1E-13   Mutation 6.1E-13
2316694 C T 17 14 7.4E-15 T 17 13 6.1E-13   Mutation 6.2E-13
414142 G A 12 11 6.7E-13 A 12 12 6.9E-15   Mutation 6.7E-13
556175 G A 13 12 4.4E-14 A 12 11 6.7E-13   Mutation 7.1E-13
355651 C T 38 30 1.8E-29 T 36 20 7.3E-13   Mutation 7.3E-13
748721 C T 14 12 2.7E-13 T 12 11 6.7E-13   Mutation 9.4E-13
2715604 C T 20 14 1.2E-12 T 24 20 4.9E-21   Mutation 1.2E-12
2504585 C T 10 10 1.6E-12 T 10 10 1.6E-12   Mutation 3.2E-12
688445 G A 12 11 6.7E-13 A 11 10 1.0E-11   Mutation 1.1E-11
222807 C T 18 16 5.8E-18 T 19 13 1.7E-11   Mutation 1.7E-11
4652102 G A 19 13 1.7E-11 A 22 20 1.3E-22   Mutation 1.7E-11
3117258 G A 25 19 2.2E-18 A 22 14 3.0E-11   Mutation 3.0E-11
1005055 C T 16 12 8.9E-12 T 14 11 2.4E-11   Mutation 3.2E-11
2264426 G A 11 10 1.0E-11 A 9 9 2.5E-11   Mutation 3.5E-11
992465 C T 14 11 2.4E-11 T 14 11 2.4E-11   Mutation 4.7E-11
1076365 G T 12 10 6.1E-11 T 12 11 6.7E-13   Mutation 6.2E-11
458994 C A 29 26 9.5E-29 A 23 14 1.4E-10   Mutation 1.4E-10
3062433 C T 18 14 4.2E-14 T 13 10 3.5E-10   Mutation 3.5E-10
421079 G T 8 8 4.0E-10 T 9 9 2.5E-11   Mutation 4.2E-10
4736812 C A 24 14 6.4E-10 A 18 16 5.8E-18   Mutation 6.4E-10
2957288 C T 12 12 6.9E-15 T 11 9 9.4E-10   Mutation 9.4E-10
3872165 C T 11 9 9.4E-10 T 10 10 1.6E-12   Mutation 9.4E-10
278409 G T 19 12 1.2E-09 T 23 14 1.4E-10   Mutation 1.4E-09
2861538 G A 15 13 1.8E-14 A 14 10 1.9E-09   Mutation 1.9E-09
1408682 G A 9 8 2.4E-09 A 9 9 2.5E-11   Mutation 2.5E-09l
272653 C A 10 9 1.6E-10 A 9 8 2.4E-09   Mutation 2.6E-09
2757635 G T 9 8 2.4E-09 T 8 8 4.0E-10   Mutation 2.8E-09
4148066 C A 9 8 2.4E-09 A 8 8 4.0E-10   Mutation 2.8E-09
206275   T 17 11 3.6E-09 T 14 11 2.4E-11   Mutation 3.6E-09
250264 C T 9 8 2.4E-09 T 9 8 2.4E-09   Mutation 4.9E-09
2425294 C T 9 8 2.4E-09 T 9 8 2.4E-09   Mutation 4.9E-09
4431921 G A 12 9 5.4E-09 A 12 10 6.1E-11   Mutation 5.4E-09
909863 C T 31 16 5.5E-09 T 31 17 9.6E-11   Mutation 5.6E-09
1085221 G A 12 9 5.4E-09 A 12 9 5.4E-09   Mutation 1.1E-08
2250730 G A 10 10 1.6E-12 A 10 8 1.5E-08   Mutation 1.5E-08
662822 G A 21 12 2.8E-08 A 19 18 4.3E-21   Mutation 2.8E-08
731542 C T 13 9 2.9E-08 T 13 11 4.1E-12   Mutation 2.9E-08
412934 G A 8 7 3.9E-08 A 10 8 1.5E-08   Mutation 5.3E-08
2104411 C T 11 8 8.2E-08 T 12 10 6.1E-11   Mutation 8.2E-08
4189314 G A 15 13 1.8E-14 A 14 9 1.5E-07   Mutation 1.5E-07
3364045 C T 14 9 1.5E-07 T 15 12 1.6E-12   Mutation 1.5E-07
2795479 C T 6 6 1.0E-07 T 6 6 1.0E-07   Mutation 2.1E-07
555449 G A 9 7 2.3E-07 A 9 9 2.5E-11   Mutation 2.3E-07
1306236 G A 12 8 4.5E-07 A 12 10 6.1E-11   Mutation 4.5E-07
4173104 G A 9 7 2.3E-07 A 9 7 2.3E-07   Mutation 4.6E-07
2260312 C T 8 7 3.9E-08 T 7 6 6.3E-07   Mutation 6.7E-07
2873628 G A 6 6 1.0E-07 A 7 6 6.3E-07   Mutation 7.4E-07
1219556 C T 9 7 2.3E-07 T 7 6 6.3E-07   Mutation 8.6E-07
3929806 C T 17 13 6.1E-13 T 18 10 1.2E-06   Mutation 1.2E-06
719703 G A 10 7 1.3E-06 A 9 8 2.4E-09   Mutation 1.3E-06
767167 C T 10 7 1.3E-06 T 11 8 8.2E-08   Mutation 1.4E-06
4671425 C T 5 5 1.7E-06 T 7 7 6.3E-09   Mutation 1.7E-06
74626 G A 5 5 1.7E-06 A 6 6 1.0E-07   Mutation 1.8E-06
1556611 G A 5 5 1.7E-06 A 6 6 1.0E-07   Mutation 1.8E-06
3771665 G A 13 8 2.3E-06 A 13 10 3.5E-10   Mutation 2.3E-06
1277370 C T 10 7 1.3E-06 T 10 7 1.3E-06   Mutation 2.6E-06
2831234 G A 8 6 3.7E-06 A 8 8 4.0E-10   Mutation 3.7E-06
4834248 G A 16 9 3.8E-06 A 17 15 8.4E-17   Mutation 3.8E-06
4640576 G A 12 10 6.1E-11 A 11 7 6.9E-06   Mutation 6.9E-06
314407 C T 11 7 6.9E-06 T 10 9 1.6E-10   Mutation 6.9E-06
1799318 G A 9 8 2.4E-09 A 6 5 1.1E-05   Mutation 1.1E-05
2647267 T C 6 5 1.1E-05 C 7 7 6.3E-09   Mutation 1.1E-05
1579929 G A 6 6 1.0E-07 A 6 5 1.1E-05   Mutation 1.1E-05
2458998 C T 6 6 1.0E-07 T 6 5 1.1E-05   Mutation 1.1E-05
3936247 C T 14 13 3.0E-15 T 14 8 1.2E-05   Mutation 1.2E-05
4121383 G A 14 8 1.2E-05 A 14 9 1.5E-07   Mutation 1.2E-05
1511517 G A 5 5 1.7E-06 A 6 5 1.1E-05   Mutation 1.2E-05
2963125 G A 5 5 1.7E-06 A 6 5 1.1E-05   Mutation 1.2E-05
2953567 G A 9 8 2.4E-09 A 9 6 2.1E-05   Mutation 2.1E-05
4521210 G A 9 6 2.1E-05 A 10 7 1.3E-06   Mutation 2.2E-05
1066165 C T 4 4 3.0E-05 T 5 5 1.7E-06   Mutation 3.2E-05
4377924 G A 5 5 1.7E-06 A 4 4 3.0E-05   Mutation 3.2E-05
655040 C T 12 7 3.6E-05 T 12 11 6.7E-13   Mutation 3.6E-05
3801057 G A 12 7 3.6E-05 A 12 8 4.5E-07   Mutation 3.6E-05
1064555 G A 4 4 3.0E-05 A 6 5 1.1E-05   Mutation 4.1E-05
3156134 C T 15 8 5.6E-05 T 12 8 4.5E-07   Mutation 5.7E-05
1090650 C T 7 5 6.1E-05 T 8 8 4.0E-10   Mutation 6.1E-05
4836541 T C 7 5 6.1E-05 C 8 8 4.0E-10   Mutation 6.1E-05
3417592 G A 7 5 6.1E-05 A 8 7 3.9E-08   Mutation 6.1E-05
3188210 G A 6 6 1.0E-07 A 7 5 6.1E-05   Mutation 6.1E-05
700494 C T 7 5 6.1E-05 T 7 6 6.3E-07   Mutation 6.2E-05
3298937 C T 7 6 6.3E-07 T 7 5 6.1E-05   Mutation 6.2E-05
496768 A G 7 5 6.1E-05 G 5 5 1.7E-06   Mutation 6.3E-05
630974 G A 7 5 6.1E-05 A 7 5 6.1E-05   Mutation 1.2E-04
4169252 G A 5 4 1.8E-04 A 6 6 1.0E-07   Mutation 1.8E-04
2123568 G A 5 4 1.8E-04 A 6 5 1.1E-05   Mutation 1.9E-04
3795698 G A 6 5 1.1E-05 A 5 4 1.8E-04   Mutation 1.9E-04
1779923 G A 7 5 6.1E-05 A 5 4 1.8E-04   Mutation 2.4E-04
3668382 G A 19 9 3.1E-04 A 20 16 2.0E-16   Mutation 3.1E-04
2989782 G A 8 5 3.4E-04 A 8 8 4.0E-10   Mutation 3.4E-04
1297655 C T 8 5 3.4E-04 T 8 7 3.9E-08   Mutation 3.4E-04
3625847 A G 8 5 3.4E-04 G 10 7 1.3E-06   Mutation 3.4E-04
4660505 G A 17 8 9.7E-04 A 20 13 8.6E-11   Mutation 9.7E-04
4586383 C T 6 4 1.0E-03 T 6 6 1.0E-07   Mutation 1.0E-03
2325510 C T 5 4 1.8E-04 T 4 3 3.2E-03   Mutation 3.4E-03
4111137 G A 5 4 1.8E-04 A 4 3 3.2E-03   Mutation 3.4E-03
2901163 C T 8 5 3.4E-04 T 7 4 5.7E-03   Mutation 6.0E-03
3551802 G A 7 4 5.7E-03 A 8 5 3.4E-04   Mutation 6.0E-03
4469079 C T 10 8 1.5E-08 T 10 5 9.1E-03   Mutation 9.1E-03
4539546 G A 5 3 0.02 A 6 4 1.0E-03   Mutation 0.02
4539738 G A 5 5 1.7E-06 A 3 2 0.06   Mutation 0.06
2955452 G A 6 3 0.10 A 7 7 6.3E-09   Mutation 0.10
4153066 G A 8 6 3.7E-06 A 6 3 0.10   Mutation 0.10
4767697 C T 6 5 1.1E-05 T 6 3 0.10   Mutation 0.10
4128014 C T 6 3 0.10 T 6 4 1.0E-03   Mutation 0.10
2410269 G A 7 3 0.23 A 8 5 3.4E-04   Mutation 0.23
3010834 C C 18 16 5.8E-18 T 15 15 2.1E-18   Mismatch 0
3615885 C C 21 21 2.1E-25 T 21 17 1.4E-17   Mismatch 0
4623405 A T 71 63 3.4E-67 A 71 66 3.6E-73   Mismatch 0
2499952 G A 15 14 2.0E-16 G 15 15 2.1E-18   Mismatch 2.2E-16
4452587 G A 10 10 1.6E-12 G 10 9 1.6E-10   Mismatch 1.6E-10
3911612 C C 21 13 4.2E-10 T 20 15 1.6E-14   Mismatch 4.2E-10
2608981 C C 7 6 6.3E-07 T 8 8 4.0E-10   Mismatch 6.3E-07
128045 G C 14 12 2.7E-13 G 12 7 3.6E-05   Mismatch 3.6E-05
3890711 G A 7 4 5.7E-03 G 7 4 5.7E-03   Mismatch 0.01
2691271 C T 7 7 6.3E-09 C or - 8 2    Damage  
2750772 G - 4 3 3.2E-03 A 5 5 1.7E-06   Damage  
3648312 A A or - 6 2   G 5 4 1.8E-04   Damage  
4329658 C T 5 4 1.8E-04 T,G,C,- 4 1    Damage  
1412330 G          original allele No  
2298627 A          original allele No  
2846790 G          original allele No  
3386508 G          original allele No  
3386511 T          original allele No  
171648 C          No mutation No  
357352 A          No mutation No  
992221 G          No mutation No  
4831490 C          No mutation No  
1291152 G          edge of map No  
3591684 C          edge of map No  
4010951 G          edge of map No  
4065759 G          edge of map No  
4314300 G          edge of map No  
  1. *Probability that the real allele is not the most dominant allele
  2. **Probability that the Judgement is not correct