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Table 1 Frequencies of inherited mutations in the offspring of ENU- or vehicle-treated father

From: Estimation of the frequency of inherited germline mutations by whole exome sequencing in ethyl nitrosourea-treated and untreated gpt delta mice

  Offspring ID   Father ID Mother ID   No. of bases sequenced in exome (depth ≥ 40) NGS-called mutations (depth ≥ 40) Confirmed mutations (by Sanger seq) Confirmed mutation frequency (×10−8/base) Average ± SD
ENU           
  99_1 Male 8 99   18,621,430 35 30 161.1  
  99_2 Male 8 99   13,301,379 42 34 255.6  
  99_4 Female 8 99   18,273,301 35 28 153.2  
  99_5 Female 8 99   18,590,333 36 31 166.8  
      Total 68,786,443 148 123 178.8 184.2 ± 47.9
Control           
  50_1 Male 43 50   6,874,190 2 2 29.1  
  50_2 Male 43 50   6,904,947 4 0 0.0  
  50_3 Female 43 50   6,743,247 2 1 14.8  
  50_4 Female 43 50   6,877,048 4 0 0.0  
      Total 27,399,432 12 3 10.9 11.0 ± 14.0