Estimation of the frequency of inherited germline mutations by whole exome sequencing in ethyl nitrosourea-treated and untreated gpt delta mice

Table 1 Frequencies of inherited mutations in the offspring of ENU- or vehicle-treated father

	Offspring ID		Father ID	Mother ID		No. of bases sequenced in exome (depth ≥ 40)	NGS-called mutations (depth ≥ 40)	Confirmed mutations (by Sanger seq)	Confirmed mutation frequency (×10⁻⁸/base)	Average ± SD
ENU
	99_1	Male	8	99		18,621,430	35	30	161.1
	99_2	Male	8	99		13,301,379	42	34	255.6
	99_4	Female	8	99		18,273,301	35	28	153.2
	99_5	Female	8	99		18,590,333	36	31	166.8
					Total	68,786,443	148	123	178.8	184.2 ± 47.9
Control
	50_1	Male	43	50		6,874,190	2	2	29.1
	50_2	Male	43	50		6,904,947	4	0	0.0
	50_3	Female	43	50		6,743,247	2	1	14.8
	50_4	Female	43	50		6,877,048	4	0	0.0
					Total	27,399,432	12	3	10.9	11.0 ± 14.0

ISSN: 1880-7062