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Table 1 Mutation spectra induced by integration of the targeting vectors

From: Mutagenic consequences of cytosine alterations site-specifically embedded in the human genome

Targeting vector TK revertants analyzed X:G-integrated revertantsa No mutation Single point mutationb Multiplee Othersf Total mutation NDg
T G A Delc Insd
pvINTC:G 457 410 (100 %) 403 (98 %) 0 0 0 2 (0.49 %) 1 (0.24 %) 0 3 (0.73 %) 6 (1.5 %) 1
pvINT5mC:G 722 676 (100 %) 667 (99 %) 3 (0.44 %) 0 0 1 (0.20 %) 1 (0.20 %) 0 4 (0.59 %) 9 (1.4 %) 0
pvINT5BrC:G 778 705 (100 %) 700 (99 %) 0 2 (0.28 %) 0 0 0 0 3 (0.43 %) 5 (0.71 %) 3
pvINTU:G 369 335 (100 %) 309 (92 %) 16 (4.8 %) 2 (0.60 %) 0 1 (0.30 %) 2 (0.60 %) 0 3 (0.90 %) 34 (8.1 %) 2
pvINTT:G 187 176 (100 %) 77 (44 %) 98 (56 %)h 0 0 0 0 0 0 98 (56 %) 1
pvINT5BrU:G 619 524 (100 %) 349 (67 %) 167 (32 %)h 4 (0.76 %) 0 1 (0.19 %) 0 2 (0.38 %) 1 (0.19 %) 175 (33 %) 2
  1. aX:G indicates C:G, 5-mC:G, 5-BrC:G, U:G, T:G, 5-BrU:G mispair
  2. bA single base substitution, one-base insertion, or one-base deletion detected at the modified base
  3. cOne-base deletion
  4. dOne-base insertion
  5. eMultiple base substitutions, deletions, and/or insertions detected at sites including the modified base in the BssSI site
  6. fMutations found at sites other than the modified base
  7. gNot detectable
  8. h P < 0.01 (significant difference versus pvINTU:G)