Mutagenic consequences of cytosine alterations site-specifically embedded in the human genome

Genes and Environment

Table 1 Mutation spectra induced by integration of the targeting vectors

Targeting vector	TK revertants analyzed	X:G-integrated revertants^a	No mutation	Single point mutation^b					Multiple^e	Others^f	Total mutation	ND^g
Targeting vector	TK revertants analyzed	X:G-integrated revertants^a	No mutation	T	G	A	Del^c	Ins^d	Multiple^e	Others^f	Total mutation	ND^g
pvINT^C:G	457	410 (100 %)	403 (98 %)	0	0	0	2 (0.49 %)	1 (0.24 %)	0	3 (0.73 %)	6 (1.5 %)	1
pvINT^5mC:G	722	676 (100 %)	667 (99 %)	3 (0.44 %)	0	0	1 (0.20 %)	1 (0.20 %)	0	4 (0.59 %)	9 (1.4 %)	0
pvINT^5BrC:G	778	705 (100 %)	700 (99 %)	0	2 (0.28 %)	0	0	0	0	3 (0.43 %)	5 (0.71 %)	3
pvINT^U:G	369	335 (100 %)	309 (92 %)	16 (4.8 %)	2 (0.60 %)	0	1 (0.30 %)	2 (0.60 %)	0	3 (0.90 %)	34 (8.1 %)	2
pvINT^T:G	187	176 (100 %)	77 (44 %)	98 (56 %)^h	0	0	0	0	0	0	98 (56 %)	1
pvINT^5BrU:G	619	524 (100 %)	349 (67 %)	167 (32 %)^h	4 (0.76 %)	0	1 (0.19 %)	0	2 (0.38 %)	1 (0.19 %)	175 (33 %)	2

^aX:G indicates C:G, 5-mC:G, 5-BrC:G, U:G, T:G, 5-BrU:G mispair
^bA single base substitution, one-base insertion, or one-base deletion detected at the modified base
^cOne-base deletion
^dOne-base insertion
^eMultiple base substitutions, deletions, and/or insertions detected at sites including the modified base in the BssSI site
^fMutations found at sites other than the modified base
^gNot detectable
^h P < 0.01 (significant difference versus pvINT^U:G)

ISSN: 1880-7062