Selective enhancing effect of metal ions on mutagenicity

Fujii, Nobuyoshi; Yano, Shigemitsu; Takeshita, Kenji

doi:10.1186/s41021-016-0049-5

Genes and Environment

Table 3 Revertant colonies of Salmonella typhimurium TA100 with metal ions and mutagens

From: Selective enhancing effect of metal ions on mutagenicity

With or without S9 mix	Mutagenic compounds (μg/plate)	Concentration of tested metal solution (M)	Number of His⁺ revertants/plate
With or without S9 mix	Mutagenic compounds (μg/plate)	Concentration of tested metal solution (M)	Ca(II)	Mg(II)	Mn(II)	Cu(II)	Zn(II)
−S9 mix	-	0	92 ± 15
	4NQO	0	266 ± 13
	(0.02)	0.0039	84^a ± 11	277 ± 27	252 ± 15	373 ± 73	233 ± 30
		0.016	99^a ± 11	236 ± 28	256 ± 23	635 ± 50	246 ± 23
		0.063	86^a ± 17	261 ± 22	157^a ± 10	619 ± 31	247 ± 14
		0.25	60^a ± 9	230 ± 23	70^a ± 8	549 ± 52	264 ± 24
	AF-2	0	334 ± 31
	(0.002)	0.0039	168^a ± 24	312 ± 12	324 ± 43	289 ± 26	286 ± 2
		0.016	136^a ± 27	280 ± 12	332 ± 48	206 ± 7	262 ± 30
		0.063	119^a ± 16	316 ± 11	240 ± 22	229 ± 18	276 ± 15
		0.25	89^a ± 12	238* ± 27	109^a ± 18	274 ± 22	280 ± 16
	Quercetin	0	235 ± 18
	(50)	0.0039	79^a ± 14	226 ± 37	233 ± 7	90^a ± 14	166 ± 23
		0.016	84^a ± 10	243 ± 19	179^a ± 15	68^a ± 10	167 ± 14
		0.063	75^a ± 3	249 ± 26	124^a ± 22	64^a ± 3	157 ± 15
		0.25	58^a ± 19	223^a ± 14	73^a ± 3	63^a ± 19	124 ± 6
+S9 mix	-	0	121 ± 39
	2-AA	0	209 ± 31
	(0.2)	0.0039	258 ± 30	218 ± 13	186 ± 16	259 ± 25	206 ± 12
		0.016	220 ± 6	229 ± 5	200 ± 16	93^a ± 7	236 ± 5
		0.063	269 ± 43	226 ± 19	227 ± 14	71^a ± 14	239 ± 19
		0.25	286^a ± 21	204^a ± 1	232 ± 10	62^a ± 6	512 ± 25
	B[a]P	0	196 ± 11
	(0.5)	0.0039	184 ± 15	188 ± 55	161 ± 20	107 ± 13	203 ± 15
		0.016	187 ± 8	169 ± 13	160 ± 9	75^a ± 19	159 ± 11
		0.063	196 ± 7	178 ± 18	180 ± 39	54^a ± 14	187 ± 25
		0.25	197^a ± 32	179 ± 15	158^a ± 15	61^a ± 33	140^a ± 30
	Trp-p-1	0	215 ± 9
	(0.1)	0.0039	197 ± 14	211 ± 31	149 ± 18	129 ± 6	167 ± 8
		0.016	206 ± 4	219 ± 43	169 ± 4	89^a ± 4	176 ± 4
		0.063	191 ± 17	195 ± 25	174 ± 18	78^a ± 20	164 ± 15
		0.25	139^a ± 2	173^a ±15	145 ± 15	73^a ± 6	146 ± 18

Values represent the mean ± SD (n = 3)
^aindicates cytotoxicity
Metal solution was used in amounts of 0.1 ml per plate

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ISSN: 1880-7062

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