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Fig. 2 | Genes and Environment

Fig. 2

From: Modulation of benzo[a]pyrene–DNA adduct formation by CYP1 inducer and inhibitor

Fig. 2

TCDD-mediated modulation of BaP adducts in various human cell lines. Hepatoma cell line HepG2 (a, b), breast carcinoma cell line MCF7 (c, d), and lung carcinoma cell line A549 (e, f) were co-treated with BaP (0.5–10 μM; a, c, e) or BPDE (0.1–5 μM; b, d, f) with or without TCDD. Closed columns represent adducts by 10 nM TCDD treatment and open columns represent adducts by solvent control (DMSO). After 16 h of incubation, genomic DNA was isolated and DNA adducts were detected by 32P-postlabeling and PAGE. G: CYP1A1 and 1B1 mRNA expression in the three cell lines. Each cell line was exposed to 10 nM TCDD or DMSO for 24 h and total RNA was extracted. For the quantitation of specific transcripts, real-time PCR was performed. These figures are adapted from data reported previously [24]

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