Fig. 1

Genotyping of the CYP2D6 rs3892097 (G/A) polymorphism by an allele-specific PCR assay and validation of genotypes by Sanger sequencing. For each sample, two PCR reactions were performed using a 1.8 kb fragment harboring rs3892097 (G/A) polymorphism as template, each including a common forward primer and allele specific reverse primers (G-Allele PCR and A-Allele PCR). A 577-bp DNA fragment indicated the presence of the G or A allele. Individuals 1 to 5 are homozygous for the G-allele, individual 6 and 7 are homozygous for the A-allele and individual 8 is heterozygous. Sanger sequencing performed on individuals with different genotypes are shown below