Radioprotective activity of blackcurrant extract evaluated by in vitro micronucleus and gene mutation assays in TK6 human lymphoblastoid cells

Table 1 Results of TK gene mutation assay in TK6 cells

Irradiation dose (Gy)	0.000	0.125	0.250	0.500	1.000
Mutant Frequency (×10⁻⁶ cells)
BCE untreated
NG mutant	2.95 ± 0.76	4.48 ± 0.84 (p < 0.05)^††	10.33 ± 6.62 (p < 0.1)^†	20.13 ± 10.28 (p < 0.1)^†	18.00 ± 6.68 (p < 0.05)^††
SG mutant	1.95 ± 0.470.47	4.61 ± 1.48 (p < 0.05)^††	5.62 ± 2.09 (p < 0.01)^†††	14.51 ± 2.57 (p < 0.01)^†††	17.74 ± 4.91 (p < 0.05)^††
Total mutant	4.90 ± 1.07	9.08 ± 1.84 (p < 0.05)^††	15.95 ± 5.21 (p < 0.05)^††	34.64 ± 11.94 (p < 0.05)^††	35.74 ± 2.04 (p < 0.01)^†††
BCE treated
NG mutant	2.38 ± 0.15	3.22 ± 0.21 (p < 0.1)*	6.69 ± 2.57 (p < 0.1)*	9.40 ± 1.59	16.20 ± 5.67
SG mutant	2.14 ± 0.13	2.63 ± 0.17 (p < 0.1)*	4.56 ± 1.04 (p < 0.1)*	6.21 ± 2.82 (p < 0.05)**	10.54 ± 3.05
Total mutant	4.68 ± 0.27	5.85 ± 0.38 (p < 0.1)*	11.25 ± 2.58 (p < 0.05)**	15.61 ± 3.13 (p < 0.1)*	26.74 ± 4.55 (p < 0.05)**

Dagger^†, daggers^†† and daggers^††† denote p < 0.1, p < 0.05 and p < 0.01, respectively, in the t-test comparing non-irradiated and γ-irradiated cells. An asterisk^* and asterisks^** denote p < 0.1 and p < 0.05, respectively, in the t-test of comparing BCE-untreated and BCE-treated cells

ISSN: 1880-7062