Fig. 2

Representation of mouse Cyp1a1 gene promoter region. The sequence is presented as reverse complement (5′ to 3′ end). Methyl-CpG at −499 from transcription start site (TSS) was indicated in HinP1 I site (blue, B′ in Fig 1). Another HinP1 I site and Nco I site were indicated as green (B in Fig. 1) and red (A in Fig. 1), respectively. The −499-CpG was 30% methylated in the control mouse liver genome DNA in our previous study [13]. The 45-bp sequence indicated by light blue character was amplified by the T7 primer and M13 reverse primer, which contain two adaptor sequences, Adaptor-3 and Adaptor-2, respectively. The PCR product containing this sequence was detected as an approximately 100-bp in Fig. 3a