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Table 2 Comet assay results for all studies

From: The effect of different methods and image analyzers on the results of the in vivo comet assay

Protocol

Analyzer

Test substance

Mean % tail DNA ± SD

Study 1

Study 2

Study 3

Method 1

Comet Assay IV

PS

2.25 ± 0.58

1.48 ± 0.36

1.71 ± 0.76

EMS

46.36 ± 5.78##

27.45 ± 11.89#

27.03 ± 1.41**

Comet Analyzer

PS

4.04 ± 0.13

3.51 ± 0.42

1.34 ± 0.18

EMS

35.12 ± 6.66##

18.54 ± 3.37##

20.40 ± 4.41##

Method 2

Comet Assay IV

PS

6.53 ± 4.34

3.39 ± 3.59

2.78 ± 1.16

EMS

21.49 ± 13.05*

12.54 ± 5.47*

25.57 ± 7.92##

Comet Analyzer

PS

4.19 ± 1.29

2.97 ± 0.52

4.37 ± 1.84

EMS

8.56 ± 1.92**

11.05 ± 3.08##

15.34 ± 4.76**

  1. Statistical analyses were conducted between the EMS-treatment and negative control groups for each of the method/analyzer combinations
  2. SD standard deviation, PS physiological saline, EMS ethyl methanesulfonate
  3. *p < 0.05, **p < 0.01 (Student’s t-test)
  4. #p < 0.05, ##p < 0.01 (Aspin-Welch’s t-test)