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Table 2 Comet assay results for all studies

From: The effect of different methods and image analyzers on the results of the in vivo comet assay

Protocol Analyzer Test substance Mean % tail DNA ± SD
Study 1 Study 2 Study 3
Method 1 Comet Assay IV PS 2.25 ± 0.58 1.48 ± 0.36 1.71 ± 0.76
EMS 46.36 ± 5.78## 27.45 ± 11.89# 27.03 ± 1.41**
Comet Analyzer PS 4.04 ± 0.13 3.51 ± 0.42 1.34 ± 0.18
EMS 35.12 ± 6.66## 18.54 ± 3.37## 20.40 ± 4.41##
Method 2 Comet Assay IV PS 6.53 ± 4.34 3.39 ± 3.59 2.78 ± 1.16
EMS 21.49 ± 13.05* 12.54 ± 5.47* 25.57 ± 7.92##
Comet Analyzer PS 4.19 ± 1.29 2.97 ± 0.52 4.37 ± 1.84
EMS 8.56 ± 1.92** 11.05 ± 3.08## 15.34 ± 4.76**
  1. Statistical analyses were conducted between the EMS-treatment and negative control groups for each of the method/analyzer combinations
  2. SD standard deviation, PS physiological saline, EMS ethyl methanesulfonate
  3. *p < 0.05, **p < 0.01 (Student’s t-test)
  4. #p < 0.05, ##p < 0.01 (Aspin-Welch’s t-test)