From: The effect of different methods and image analyzers on the results of the in vivo comet assay
Protocol | Analyzer | Test substance | Mean % tail DNA ± SD | ||
---|---|---|---|---|---|
Study 1 | Study 2 | Study 3 | |||
Method 1 | Comet Assay IV | PS | 2.25 ± 0.58 | 1.48 ± 0.36 | 1.71 ± 0.76 |
EMS | 46.36 ± 5.78## | 27.45 ± 11.89# | 27.03 ± 1.41** | ||
Comet Analyzer | PS | 4.04 ± 0.13 | 3.51 ± 0.42 | 1.34 ± 0.18 | |
EMS | 35.12 ± 6.66## | 18.54 ± 3.37## | 20.40 ± 4.41## | ||
Method 2 | Comet Assay IV | PS | 6.53 ± 4.34 | 3.39 ± 3.59 | 2.78 ± 1.16 |
EMS | 21.49 ± 13.05* | 12.54 ± 5.47* | 25.57 ± 7.92## | ||
Comet Analyzer | PS | 4.19 ± 1.29 | 2.97 ± 0.52 | 4.37 ± 1.84 | |
EMS | 8.56 ± 1.92** | 11.05 ± 3.08## | 15.34 ± 4.76** |