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Table 1 Accuracy and precision data (n = 8)

From: Simultaneous and absolute quantification of nucleoside triphosphates using liquid chromatography–triple quadrupole tandem mass spectrometry

Analyte

Expected concentration (nM)

Calculated concentration (nM)

%CV

%Bias

dATP

10

10.0

4.0

0.4

100

100.9

1.2

0.9

1000

987.8

1.4

−1.2

dGTP

10

10.1

5.4

1.2

100

100.4

3.3

0.4

1000

969.3

2.9

−3.1

dCTP

10

9.8

3.3

−1.6

100

102.0

0.6

2.0

1000

1000.5

1.6

0.1

dTTP

10

10.2

2.0

2.0

100

99.5

1.5

−0.5

1000

983.8

3.2

−1.6

ATP

1000

1004.5

2.0

0.5

10,000

10,068.8

0.9

0.7

100,000

98,628.5

1.4

−1.4

GTP

1000

1004.3

1.1

0.4

10,000

10,197.3

2.0

2.0

100,000

98,085.3

1.9

−1.9

CTP

1000

1002.0

1.1

0.2

10,000

10,060.3

1.4

0.6

100,000

98,854.1

0.8

−1.1

UTP

1000

1006.3

2.3

0.6

10,000

10,252.5

2.8

2.5

100,000

100,137.9

2.5

0.1