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Fig. 2 | Genes and Environment

Fig. 2

From: Structure analysis of yeast glutaredoxin Grx6 protein produced in Escherichia coli

Fig. 2

ScGrx6 holoprotein stabilization in aerobic solutions by the addition of DTT, EDTA, GSH, GSSG or H2O2 at 2 mM. The absorbance of freshly purified ScGrx6 at 430 nm was measured at six different time points in the absence and presence of DTT, EDTA, GSH, GSSG or H2O2. The absorbance of the buffer containing 20 mM sodium chloride and 50 mM tris hydrochloride, pH 8.0, was subtracted using a reference cuvette. All assays were performed at 25 °C. The concentration of ScGrx6 in the assays was 2 μM. Time points: 0, 50, 100, 150, 200 and 250 min; blank is free of all additives. The experiment was repeated three times

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