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Fig. 2 | Genes and Environment

Fig. 2

From: Collaborative study of thresholds for mutagens: proposal of a typical protocol for detection of hormetic responses in cytotoxicity tests

Fig. 2

Preliminary experimental results. CHL/IU (a, b, and c), TK6 (e and d) or HeLa S3 (f) cells were treated with mitomycin C two days after inoculation for 24 h and OD-450 was measured 2 h after addition of CCK-8. Data show OD-450 ratios (Mean ± SD). Lines connecting between the zero dose and the highest dose depict dose-response relationship expected when applying the LNT hypothesis. Although the scale of the x-axis is neither linear nor logarithmic, it is closer to logarithmic; expectations from LNT are closer to curved lines as shown in Fig. 1c and d. When the scale of the x-axis is linear, presentation of all data in a single graph is difficult. Therefore, responses within a small range (from 0 to 1 ng/mL) are depicted on a linear scale in Fig. 2a (insert), in which the blue line shows an LNT response. The number of wells were three for a and f, four for b, d, and e, and six for e. In e, the outer 36 wells were used as medium blanks, and the mean reading was subtracted from experimental readings. The control in E consisted of 12 wells

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