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Fig. 3 | Genes and Environment

Fig. 3

From: Collaborative study of thresholds for mutagens: proposal of a typical protocol for detection of hormetic responses in cytotoxicity tests

Fig. 3

Number of cells per well and hormetic expression time. Firstly, different numbers of HeLa S3 cells (2000, 4000, and 6000 cells/well) were treated with EMS for 6 (a), 12 (b), and 24 h (c) two days after inoculation. Secondly, Hela S3 cells (500, 1000, and 2000 cells/well) were treated with EMS for 9 (d), 12 (e), and 15 h (f). CCK-8 (10 μL/well) was added 2 h before OD-450 measurement. Data show OD-450 ratios (Mean ± SD)

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