Skip to main content
Fig. 5 | Genes and Environment

Fig. 5

From: Collaborative study of thresholds for mutagens: proposal of a typical protocol for detection of hormetic responses in cytotoxicity tests

Fig. 5

Characteristics of CCK-8 coloration. HeLa S3 cells (2000 cells per well) were plated and treated with nine concentrations (5 to 2000 ng/mL as shown at the right of the graph) two days after inoculation. CCK-8 (5 μL/well) was added 10 h after addition of MMC and OD-450 was measured 11, 12, 13, 14, 15 and 21 h after the addition of MMC (1, 2, 3, 4, 5, and 11 h after the addition of CCK-8, respectively)

Back to article page