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Table 1 Convection effect. CHL/IU cells were inoculated in wells of a 96-well plate (5000 cells/100 μL) or of a 48-well plate (10,000 cells/200 μL). After a two-day culturing period, 10 μL (96-well plate) or 20 μL (48-well plate) of CCK-8 were added to each well and OD-450 was measured 2 h after the addition of CCK-8

From: Collaborative study of thresholds for mutagens: proposal of a typical protocol for detection of hormetic responses in cytotoxicity tests

Plates

Standing time (min)

OD-450 (mean ± SD, n = 6)

96-well

8

0.826 ± 0.116

 

30

1.085 ± 0.143

48-well

0

0.668 ± 0.022

 

30

0.742 ± 0.004