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Fig. 3 | Genes and Environment

Fig. 3

From: Oligo swapping method for in vitro DNA repair substrate containing a single DNA lesion at a specific site

Fig. 3

Preparation of DNA template (8-oxoG substrate) with 8-oxoG at a defined position. a Upper strand sequence containing 8-oxoG base and lower strand substrate containing original C base in pBS2/8-oxoG are shown diagrammatically. b Experimental procedure for purification of pBS2/8-oxoG using 8-oxoG oligo. c Identified DNA substrates (100 ng), pBS2/8-oxoG, were incubated with Fpg (2 units) or hOGG1 (0.16 units) at 37 °C for 30 min. Aliquots from the sample were run on 0.8% agarose gel and visualized by staining with EtBr. Lane 1, 1-kbp marker; lane 2, non-treatment; lane 3, Fpg-treatment; lane 4, hOGGI-treatment. Open circular DNA (OC), liner DNA (Lin), and covalently closed circular DNA (CCC) are indicated by arrows

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Genes and Environment

ISSN: 1880-7062

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