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Fig. 4 | Genes and Environment

Fig. 4

From: Oligo swapping method for in vitro DNA repair substrate containing a single DNA lesion at a specific site

Fig. 4

Preparation of DNA template (uracil substrate) with uracil at a defined position. a Upper strand sequence containing uracil base and lower strand substrate containing original C base in pBS2/Uracil are shown diagrammatically. b Experimental procedure for purification of pBS2/uracil using uracil oligo. c Identified DNA substrates (100 ng), pBS2/uracil, were incubated with UDG (0.5 units) or UDG (0.5 units) + APE1 (5 units) at 37 °C for 30 min. Aliquots from the sample were analyzed on 0.8% agarose gel, and the DNA substrates were visualized by staining with EtBr. Lane 1, 1-kbp marker; lane 2, non-treatment; lane 3, UDG-treatment; lane 4, UDG + APE1-treatment. Open circular DNA (OC), liner DNA (Lin), and covalently closed circular DNA (CCC) are indicated by arrows

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Genes and Environment

ISSN: 1880-7062

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