Methods for MN detection | Advantage | Limitation | Reference |
---|---|---|---|
Imaging analysis with slide glass specimens | Easy to confirm the validity of a MN-detection algorithm by means of checking the MN images manually by skilled observers. | Limited to middle-throughput.Difficult to apply multi-endpoint assays. | 16 |
Difficult to apply robotics for plate preparation. | |||
Imaging analysis with micro-plates in adherent cells | Easy to confirm the validity of an algorithm by means of checking the MN images manually by skilled observers. | Difficult to apply to non-adherent cells such as TK6 or primary lymphocytes. | 10, 11 |
Easy to apply robotics for plate preparation. | |||
Flowcytometric analysis | Easy to apply to non-adherent cells such as TK6 or primary lymphocytes. Applicable to multi-endpoint assay (e.g. gH2AX, H3, p53). | Difficult to confirm whether the MN detected were bona fide. | 18 |
Difficult to apply robotics for the sample preparation. | |||
Imaging analysis with micro-plate in non-adherent cells (the method in this article) | Easy to confirm the validity of an algorithm by means of checking the MN images manually by skilled observers. | Difficult to apply robotics for plate preparation. | This article |
Applicable to multi-endpoint assay (e.g. gH2AX). |