Fig. 2
From: Mechanism and regulation of DNA damage recognition in nucleotide excision repair
Stimulation of in vitro dual incision reactions with CPD substrates by the presence of mismatched bases or an AP site. Internally 32P-labeled DNA substrates containing a site-specific CPD (~ 200 bp in length) were incubated with six purified recombinant NER factors, and excised oligonucleotides containing both the 32P label and CPD were detected by denaturing polyacrylamide gel electrophoresis and the following autoradiography as described previously [12, 50]