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Fig. 6 | Genes and Environment

Fig. 6

From: Purification and interactions of the MucA’ and MucB proteins constituting the DNA polymerase RI

Fig. 6

The effect of MucA’ on the binding of MucB to ssDNA. MucB (0.26 μM) alone or MucB (0.26 μM) premixed with MucA’ (1 μM or 10 μM, i.e. low or high, as indicated) proteins were injected over the flow cells of SA chip with immobilized 73-mer single- stranded oligonucleotide, double-stranded oligonucleotide and the control blank cell without DNA. When the binding to double stranded oligonucleotide was examined, the 73-mer single stranded oligonucleotide was first annealed with the complementary strand and then the annealed oligonucleotide was bound on the SA chip surface via streptavidin/biotin interaction. a Comparison of sensograms demonstrating the inhibitory effect of MucA’ on MucB binding to single stranded oligonucleotide. b Sensograms showing the kinetics of MucB binding to ssDNA and dsDNA. The presented data are curves adjusted by subtracting the response curve in the control blank cell streptavidin surface from those curves in the test flow cells using the Pharmacia Biosensor kinetics software (BIAevaluation 2.1) and therefore represent the DNA specific responses

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