TY - JOUR AU - Ishida, Takenori AU - Fujihara, Nobutoshi AU - Nishimura, Tomoki AU - Funabashi, Hisakage AU - Hirota, Ryuichi AU - Ikeda, Takeshi AU - Kuroda, Akio PY - 2019 DA - 2019/06/05 TI - Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy JO - Genes and Environment SP - 14 VL - 41 IS - 1 AB - Frustrated phagocytosis occurs when an asbestos fiber > 10 μm in length is engulfed imperfectly by a macrophage, and it is believed to be associated with chromosomal instability. Few studies have focused on dynamic cellular imaging to assess the toxicity of hazardous inorganic materials such as asbestos. One reason for this is the relative lack of fluorescent probes available to facilitate experimental visualization of inorganic materials. We recently developed asbestos-specific fluorescent probes based on asbestos-binding proteins, and achieved efficient fluorescent labeling of asbestos. SN - 1880-7062 UR - https://doi.org/10.1186/s41021-019-0129-4 DO - 10.1186/s41021-019-0129-4 ID - Ishida2019 ER -