Fig. 3

Confocal laser scanning microscopy images of phagocytosis of fluorescently labeled asbestos fibers by RAW 264.7 cells. RAW 264.7 cells were incubated with fluorescently labeled crocidolite (red) (a, b) and amosite (red) (c) asbestos for 2 h. The actin cytoskeleton and nuclei of the cells were respectively stained with ActinGreen™ 488 (green) and DAPI (blue). Z-axis images at vertical and horizontal yellow lines were extracted from 3D images, and respectively indicate right and bottom positions. Scale bar = 10 μm