Fig. 2

Generation of XPC^−/− and CSB^−/− cells. a Schematic representation of the targeted disruption of XPC and CSB. The target sequence of CRISPR/Cas9 and the targeting vector containing a neomycin-resistance (neo^R) or hygromycin-resistance (hygro^R) marker cassette in the opposite direction are shown. The black boxes and triangles represent the exons and loxP sequences, respectively. b Western blot analysis for the XPC and CSB proteins. Whole cell extracts of WT, XPC^−/−, and CSB^−/− were loaded onto a 10% SDS-polyacrylamide gel. α-Tubulin served as a loading control. c RT-PCR analysis for CSB mRNA. The same amounts of total RNA extracted from each cell were used. β-Actin served as an internal control. d The sequence of CSB cDNA generated by RT-PCR. The sequences around codon 337 are shown