Fig. 4
From: Development of a novel PIG-A gene mutation assay based on a GPI-anchored fluorescent protein sensor
Depletion of pre-existing GPI(−) TK6 cells by repopulation. Flow cytometry was performed using an LSRFortessa (BSL2). GFP/mCherry double positive GPI(+) cells located in Q2, and GFP/mCherry double negative GPI(−) cells located in Q4. a GPI(−) cells and b GPI(+) cells before repopulation of GPI(+) cells using fluorescence-activated cell sorter, c, d two GPI(+) cells after repopulation of GPI(+) cells using fluorescence-activated cell sorter