Fig. 2

Measurement of MN frequency in human HepaRG™ cells following exposure to: (a) DDI chemicals in μM concentrations; and (b) non-DDI chemicals in mM concentrations (except COL, which was in μM) using the In Vitro MicroFlow® assay (Litron Laboratories). Percentage of MN induction is depicted 96 h following the last exposure (n = 2). See Table 1 for specific concentrations (C1 = lowest concentration and C6 = highest concentration). DDI chemical abbreviations: 2-nitrofluorene (2NF), cisplatin (CISP), etoposide (ETP), aflatoxin B1 (AFB1), and methyl methanesulfonate (MMS). Non-DDI chemical abbreviations: 2-deoxy-D-glucose (2DG), sodium chloride (NaCl), ampicillin trihydrate (AMP), sodium ascorbate (ASC), and colchicine (COL). Control represents the vehicle control (DMSO for 2NF, CISP, ETP, AFB1, and COL; water for MMS; media for 2DG, NaCl, AMP, and ASC). Error bars depict standard error, but are too small to see for many data points. * P < 0.01 compared to the vehicle control