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Table 1 Test Chemical Information

From: Flow cytometric micronucleus assay and TGx-DDI transcriptomic biomarker analysis of ten genotoxic and non-genotoxic chemicals in human HepaRG™ cells

Test Chemical

Chemical Abbreviation

CAS No.

Vehicle Control

Concentrations Tested

DNA Damage-Inducing (DDI) Chemicals

 Aflatoxin B1

AFB1

1162-65-8

DMSO

0.1, 0.25, 0.5, 1, 2.5, 5* μM

 Cisplatin

CISP

15,663–27-1

DMSO

1, 2, 3, 5, 10, 20* μM

 Etoposide

ETP

33,419–42-0

DMSO

0.25, 0.5, 1, 2.5, 5, 10 μM

 Methyl methanesulfonate

MMS

66–27-3

Water

5, 10, 20, 50, 100, 200 μM

 2-Nitrofluorene

2NF

607–57-8

DMSO

2, 10, 50, 100, 250, 500* μM

Non-DNA Damage-Inducing (Non-DDI) Chemicals

 Ampicillin Trihydrate

AMP

7177-48-2

Media

0.4, 1, 2, 3, 5, 10 mM

 Colchicine

COL

64–86-8

DMSO

0.0125, 0.025, 0.05, 0.1, 0.2, 0.3 μM

 2-Deoxy-D-Glucose

2DG

154–17-6

Media

0.3125, 0.625, 1.25, 2.5, 5, 10 mM

 Sodium Ascorbate

ASC

134–03-2

Media

0.1, 0.4, 1, 2, 4, 10 mM

 Sodium Chloride

NaCl

7647-14-5

Media

0.5, 1, 2.5, 5, 7.5, 10 mM

  1. *indicates a cytotoxic concentration (< 40% RS) that was subsequently eliminated from the analysis; concentrations that are underlined were used for gene expression analysis (a low, mid and high concentration were selected based on criteria in Buick et al. (2015) and Buick et al. (2017) using the % RS and % MN data).