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Table 1 Test Chemical Information

From: Flow cytometric micronucleus assay and TGx-DDI transcriptomic biomarker analysis of ten genotoxic and non-genotoxic chemicals in human HepaRG™ cells

Test ChemicalChemical AbbreviationCAS No.Vehicle ControlConcentrations Tested
DNA Damage-Inducing (DDI) Chemicals
 Aflatoxin B1AFB11162-65-8DMSO0.1, 0.25, 0.5, 1, 2.5, 5* μM
 CisplatinCISP15,663–27-1DMSO1, 2, 3, 5, 10, 20* μM
 EtoposideETP33,419–42-0DMSO0.25, 0.5, 1, 2.5, 5, 10 μM
 Methyl methanesulfonateMMS66–27-3Water5, 10, 20, 50, 100, 200 μM
 2-Nitrofluorene2NF607–57-8DMSO2, 10, 50, 100, 250, 500* μM
Non-DNA Damage-Inducing (Non-DDI) Chemicals
 Ampicillin TrihydrateAMP7177-48-2Media0.4, 1, 2, 3, 5, 10 mM
 ColchicineCOL64–86-8DMSO0.0125, 0.025, 0.05, 0.1, 0.2, 0.3 μM
 2-Deoxy-D-Glucose2DG154–17-6Media0.3125, 0.625, 1.25, 2.5, 5, 10 mM
 Sodium AscorbateASC134–03-2Media0.1, 0.4, 1, 2, 4, 10 mM
 Sodium ChlorideNaCl7647-14-5Media0.5, 1, 2.5, 5, 7.5, 10 mM
  1. *indicates a cytotoxic concentration (< 40% RS) that was subsequently eliminated from the analysis; concentrations that are underlined were used for gene expression analysis (a low, mid and high concentration were selected based on criteria in Buick et al. (2015) and Buick et al. (2017) using the % RS and % MN data).