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Table 2 MN frequency and TGx-DDI classification for test chemicals using NSC probability analysis, principal component analysis, and 2-dimensional clustering

From: Flow cytometric micronucleus assay and TGx-DDI transcriptomic biomarker analysis of ten genotoxic and non-genotoxic chemicals in human HepaRG™ cells

Test ChemicalMN InductionOverall TGx-DDI ClassificationPA ClassificationPCA Classification2 DC Classification
LowMidHighLowMidHighLowMidHighLowMidHighLowMidHigh
DDI Chemicals
 Aflatoxin B1+++Non-DDIDDIDDINon-DDIDDIDDINon-DDIDDIDDINon-DDINon-DDIDDI
 Cisplatin++Non-DDINon-DDIDDINon-DDINon-DDIDDINon-DDINon-DDIDDINon-DDINon-DDINon-DDI
 Etoposide+++DDIDDIDDIDDIDDIDDINon-DDIDDIDDINon-DDINon-DDINon-DDI
 Methyl methanesulfonate++Non-DDIDDIDDINon-DDIDDIDDINon-DDIDDIDDINon-DDIDDIDDI
 2-Nitrofluorene++Non-DDIDDIDDINon-DDIDDIDDINon-DDIDDIDDINon-DDIDDIDDI
Non-DDI Chemicals
 Ampicillin TrihydrateNon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDI
 Colchicine++Non-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDI
 2-Deoxy-D-GlucoseNon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDI
 Sodium AscorbateNon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDI
 Sodium ChlorideNon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDINon-DDI
  1. For MN Induction, a ‘+’ sign indicates a statistically significant induction of MN and at least a two-fold change over vehicle controls and a ‘-‘sign indicates that there was no statistically significant induction of MN. For the TGx-DDI classification results, DDI represents a DNA damage-inducing classification; whereas, non-DDI represents a non-DNA damage-inducing classification. The ‘overall’ DDI call requires a DDI call in at least one of the three analyses (PA, 2 DC, PCA), whereas a non-DDI is non-DDI in all of these analyses