Fig. 1
From: Effects of acetaldehyde-induced DNA lesions on DNA metabolism
Endonucleolytic digestion of acetaldehyde-treated plasmids. a In the absence of DNA damage, the indicated restriction enzymes generated digested DNA fragments from EcoRI-digested pBS DNA templates. If acetaldehyde induced damages GG sequences in DNA are present, the resulting GG lesions are resistant to digestion by restriction enzymes, and full-digested DNA fragments will not be detected on agarose gel. b Agarose gel (1%) demonstrating the presence of the GG lesion. EcoRI-digested pBSII DNA templates (lanes 1 and 2) were digested with restriction enzymes MluCI (lanes 3 and 4), HaeIII (lanes 5 and 6), MspI (lanes 7 and 8) and HhaI (lanes 9 and 10). Acetaldehyde-treated EcoRI-digested pBSII DNA templates (lanes 2, 4, 6, 8, and 10). The mobility of the linear (lin) EcoRI-digested pBSII DNA templates and the covalently closed circular (ccc) undigested pBSII DNA templates is indicated along the side of the gel