Fig. 1From: High-throughput analysis of DNA repair in microplates towards identification of inhibitorsThe principle of the uracil DNA glycosylase assay. The substrate oligodeoxyribonucleotide is fixed to the surface of a microplate and bears a uracil base (shown in red) and fluorescein (shown as F) at the 5′-end. The action of glycosylase a forms an abasic site, and subsequent alkaline denaturation b induces the backbone cleavage and loss of fluoresceinBack to article page