Strain or plasmid | Description | Source |
---|---|---|
Strains | ||
TA1535 | hisG46, gal, Δ (chl, uvrB, bio), rfa | [1] |
TA100 | As TA1535, but harbors pKM101; Apr | [1] |
YG5151 | As TA1535, but ΔumuDCST::Kmr, ΔsamAB::Cmr | This study, [23] |
YG9056 | As YG5151, but harbors pKM101; + Apr | This study |
YG9028 | As YG5151, but harbors pYG8524 + pYG8517; + Apr + Tcr | This study, [23] |
YG9029 | As YG5151, but harbors pYG8529 + pYG8518; + Apr + Tcr | This study |
YG9032 | As YG5151, but harbors pYG8524 + pYG8518; + Apr + Tcr | This study |
YG9033 | As YG5151, but harbors pYG8529 + pYG8517; + Apr + Tcr | This study |
Plasmids | ||
pKM101 | R46 derivative carrying the mucAB operon of Shigella sonnei | [7] |
pYG8524 | pBR322 derivative expressing the activated MucA’ subunit of PolRI from the lac promoter | [19] |
pYG8529 | pBR322 derivative expressing the activated SamA’ polymerase subunit from the lac promoter | [19] |
pYG8517 | pSC101 derivative expressing the MucB catalytic subunit of PolRI from the lac promoter | [19] |
pYG8518 | pSC101 derivative expressing the SamB polymerase subunit from the lac promoter | [19] |