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Table 1 Characteristics of S. typhimurium strains and plasmids used in the study. Plasmids expressing Y-family DNA polymerase catalytical and accessory subunits incorporated in the Ames test strains used in present study are shown in the lower part

From: Effect of episomally encoded DNA polymerases on chemically induced mutagenesis at the hisG46 target in Ames test

Strain or plasmid

Description

Source

Strains

 TA1535

hisG46, gal, Δ (chl, uvrB, bio), rfa

[1]

 TA100

As TA1535, but harbors pKM101; Apr

[1]

 YG5151

As TA1535, but ΔumuDCST::Kmr, ΔsamAB::Cmr

This study, [23]

 YG9056

As YG5151, but harbors pKM101; + Apr

This study

 YG9028

As YG5151, but harbors pYG8524 + pYG8517; + Apr + Tcr

This study, [23]

 YG9029

As YG5151, but harbors pYG8529 + pYG8518; + Apr + Tcr

This study

 YG9032

As YG5151, but harbors pYG8524 + pYG8518; + Apr + Tcr

This study

 YG9033

As YG5151, but harbors pYG8529 + pYG8517; + Apr + Tcr

This study

Plasmids

 pKM101

R46 derivative carrying the mucAB operon of Shigella sonnei

[7]

 pYG8524

pBR322 derivative expressing the activated MucA’ subunit of PolRI from the lac promoter

[19]

 pYG8529

pBR322 derivative expressing the activated SamA’ polymerase subunit from the lac promoter

[19]

 pYG8517

pSC101 derivative expressing the MucB catalytic subunit of PolRI from the lac promoter

[19]

 pYG8518

pSC101 derivative expressing the SamB polymerase subunit from the lac promoter

[19]

  1. Kmr resistant to kanamycin, Cmr resistant to chloramphenicol, Tcr resistant to tetracycline, Apr resistant to ampicillin