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Table 1 Characteristics of S. typhimurium strains and plasmids used in the study. Plasmids expressing Y-family DNA polymerase catalytical and accessory subunits incorporated in the Ames test strains used in present study are shown in the lower part

From: Effect of episomally encoded DNA polymerases on chemically induced mutagenesis at the hisG46 target in Ames test

Strain or plasmidDescriptionSource
Strains
 TA1535hisG46, gal, Δ (chl, uvrB, bio), rfa[1]
 TA100As TA1535, but harbors pKM101; Apr[1]
 YG5151As TA1535, but ΔumuDCST::Kmr, ΔsamAB::CmrThis study, [23]
 YG9056As YG5151, but harbors pKM101; + AprThis study
 YG9028As YG5151, but harbors pYG8524 + pYG8517; + Apr + TcrThis study, [23]
 YG9029As YG5151, but harbors pYG8529 + pYG8518; + Apr + TcrThis study
 YG9032As YG5151, but harbors pYG8524 + pYG8518; + Apr + TcrThis study
 YG9033As YG5151, but harbors pYG8529 + pYG8517; + Apr + TcrThis study
Plasmids
 pKM101R46 derivative carrying the mucAB operon of Shigella sonnei[7]
 pYG8524pBR322 derivative expressing the activated MucA’ subunit of PolRI from the lac promoter[19]
 pYG8529pBR322 derivative expressing the activated SamA’ polymerase subunit from the lac promoter[19]
 pYG8517pSC101 derivative expressing the MucB catalytic subunit of PolRI from the lac promoter[19]
 pYG8518pSC101 derivative expressing the SamB polymerase subunit from the lac promoter[19]
  1. Kmr resistant to kanamycin, Cmr resistant to chloramphenicol, Tcr resistant to tetracycline, Apr resistant to ampicillin