Effects of the scid mutation on X-ray-induced deletions in the brain and spleen of gpt delta mice

Genes and Environment

Table 1 Spi⁻ mutation spectra in the brain of X-ray-irradiated WT and the scid mice

	WT 0 Gy			WT 10 Gy			scid 0 Gy			scid 10 Gy
	No.	%	MFx10⁻⁶	No.	%	MFx10⁻⁶	No.	%	MFx10⁻⁶	No.	%	MFx10⁻⁶
1 bp deletion
In run	18	72	0.74	30	42	2.08	6	75	0.64	40	42	1.22
Other	2	8	0.08	6	8	0.42	0	0	0.00	10	11	0.30
> 2 bp deletion
With microhomology	0	0	0.00	21	29	1.46	0	0	0.00	25	26	0.76
Without microhomology	3	12	0.12	12	17	0.83	1	13	0.11	11	12	0.33
Insertion	0	0	0.00	2	3	0.14	0	0	0.00	4	4	0.12
Insertion	2	8	0.08	0	0	0.00	0	0	0.00	5	5	0.15
Complex	0	0	0.00	1	1	0.07	1	13	0.11	0	0	0.00
Total	25	100	1.03	72	100	5.00	8	100	0.85	95	100	2.89

Specific MFs were calculated by multiplying the total MF by the ratio of each class of mutants
In unirradiated WT mice, total 26 mutants (suppl. Table 1) were obtained. They were sequenced and 25 deletion mutations (Table 1) were identified. In 10 Gy-irradiated WT mice, total 172 mutants were obtained. Eighty seven mutants were sequenced and 72 deletion mutations were identified. In unirradiated scid mice, total 9 mutants were obtained. All were sequenced and 8 deletion mutations were identified. In 10 Gy-irradiated scid mice, total 67 + 89 mutants were obtained. One hundred and twenty five were sequenced and 95 deletion mutations were identified. In 10 Gy-irradiated scid mice, 89 mutants were obtained by additional plating for sequencing analysis, but they were not used for calculation of MF because no additional plating was conducted for control groups

ISSN: 1880-7062