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Table 1 Spi mutation spectra in the brain of X-ray-irradiated WT and the scid mice

From: Effects of the scid mutation on X-ray-induced deletions in the brain and spleen of gpt delta mice

  WT 0 Gy WT 10 Gy scid 0 Gy scid 10 Gy
No. % MFx10−6 No. % MFx10−6 No. % MFx10−6 No. % MFx10−6
1 bp deletion
 In run 18 72 0.74 30 42 2.08 6 75 0.64 40 42 1.22
 Other 2 8 0.08 6 8 0.42 0 0 0.00 10 11 0.30
> 2 bp deletion
 With microhomology 0 0 0.00 21 29 1.46 0 0 0.00 25 26 0.76
 Without microhomology 3 12 0.12 12 17 0.83 1 13 0.11 11 12 0.33
 Insertion 0 0 0.00 2 3 0.14 0 0 0.00 4 4 0.12
Insertion 2 8 0.08 0 0 0.00 0 0 0.00 5 5 0.15
Complex 0 0 0.00 1 1 0.07 1 13 0.11 0 0 0.00
Total 25 100 1.03 72 100 5.00 8 100 0.85 95 100 2.89
  1. Specific MFs were calculated by multiplying the total MF by the ratio of each class of mutants
  2. In unirradiated WT mice, total 26 mutants (suppl. Table 1) were obtained. They were sequenced and 25 deletion mutations (Table 1) were identified. In 10 Gy-irradiated WT mice, total 172 mutants were obtained. Eighty seven mutants were sequenced and 72 deletion mutations were identified. In unirradiated scid mice, total 9 mutants were obtained. All were sequenced and 8 deletion mutations were identified. In 10 Gy-irradiated scid mice, total 67 + 89 mutants were obtained. One hundred and twenty five were sequenced and 95 deletion mutations were identified. In 10 Gy-irradiated scid mice, 89 mutants were obtained by additional plating for sequencing analysis, but they were not used for calculation of MF because no additional plating was conducted for control groups