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Table 1 Comparison of responses to endogenous agonist ligands among wild-type and various mutant yeast strains expressing sex steroid hormone receptors

From: Construction of reporter gene assays using CWP and PDR mutant yeasts for enhanced detection of various sex steroids

Ligand E1
Yeast strain ERα W303a ERα cwp1Δcwp2Δ ERβ W303a ERβ cwp1Δcwp2Δ
Fold difference (10−3 μM) 1 10.9 1 4.3
Detection limit (μM) 10−4 10−5 10− 4 10− 5
EC50 (μM) 1.20 × 10− 4 2.28 × 10− 5 2.51 × 10− 4 1.50 × 10− 5
Ligand E2
Yeast strain ERα W303a ERα cwp1Δcwp2Δ ERβ W303a ERβ cwp1Δcwp2Δ
Fold difference (10−3 μM) 1 21.7 1 5.7
Detection limit (μM) 10−4 10−5 10− 4 10− 5
EC50 (μM) 1.84 × 10− 4 2.23 × 10− 5 1 .42 × 10− 4 3.65 × 10− 5
Ligand E3
Yeast strain ERα W303a ERα cwp1Δcwp2Δ ERβ W303a ERβ cwp1Δcwp2Δ
Fold difference (1 μM) 1 10.1 1 2.9
Detection limit (μM) 0.01 10−5 0.1 10−4
EC50 (μM) 0.15 3.02 × 10−5 0.034 1.22 × 10−3
Ligand PS
Yeast strain PR W303a PR cwp1Δpdr5Δ PR cwp1Δcwp2Δ
Fold difference (10 μM) 1 34.2 29.4
Detection limit (μM) 10 0.01 0.01
EC50 (μM) 2.31 0.08 0.15
Ligand TS
Yeast strain AR W303a AR cwp1Δpdr5Δ AR pdr5Δpdr10Δ
Fold difference (1 μM) 1 5.2 3.7
Detection limit (μM) 0.01 0.01 0.01
EC50 (μM) 0.024 0.021 0.013
Ligand DHT
Yeast strain AR W303a AR cwp1Δpdr5Δ AR pdr5Δpdr10Δ
Fold difference (100 μM) 1 14.4 18.6
Detection limit (μM) 0.01 0.01 0.01
EC50 (μM) 0.028 0.011 0.023
  1. Abbreviation used: EC50, 50% effective concentration
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Genes and Environment

ISSN: 1880-7062

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