Table 1 Comparison of responses to endogenous agonist ligands among wild-type and various mutant yeast strains expressing sex steroid hormone receptors
Ligand | E1 | |||
|---|---|---|---|---|
Yeast strain | ERα W303a | ERα cwp1Δcwp2Δ | ERβ W303a | ERβ cwp1Δcwp2Δ |
Fold difference (10−3 μM) | 1 | 10.9 | 1 | 4.3 |
Detection limit (μM) | 10−4 | 10−5 | 10− 4 | 10− 5 |
EC50 (μM) | 1.20 × 10− 4 | 2.28 × 10− 5 | 2.51 × 10− 4 | 1.50 × 10− 5 |
Ligand | E2 | |||
Yeast strain | ERα W303a | ERα cwp1Δcwp2Δ | ERβ W303a | ERβ cwp1Δcwp2Δ |
Fold difference (10−3 μM) | 1 | 21.7 | 1 | 5.7 |
Detection limit (μM) | 10−4 | 10−5 | 10− 4 | 10− 5 |
EC50 (μM) | 1.84 × 10− 4 | 2.23 × 10− 5 | 1 .42 × 10− 4 | 3.65 × 10− 5 |
Ligand | E3 | |||
Yeast strain | ERα W303a | ERα cwp1Δcwp2Δ | ERβ W303a | ERβ cwp1Δcwp2Δ |
Fold difference (1 μM) | 1 | 10.1 | 1 | 2.9 |
Detection limit (μM) | 0.01 | 10−5 | 0.1 | 10−4 |
EC50 (μM) | 0.15 | 3.02 × 10−5 | 0.034 | 1.22 × 10−3 |
Ligand | PS | |||
Yeast strain | PR W303a | PR cwp1Δpdr5Δ | PR cwp1Δcwp2Δ | |
Fold difference (10 μM) | 1 | 34.2 | 29.4 | |
Detection limit (μM) | 10 | 0.01 | 0.01 | |
EC50 (μM) | 2.31 | 0.08 | 0.15 | |
Ligand | TS | |||
Yeast strain | AR W303a | AR cwp1Δpdr5Δ | AR pdr5Δpdr10Δ | |
Fold difference (1 μM) | 1 | 5.2 | 3.7 | |
Detection limit (μM) | 0.01 | 0.01 | 0.01 | |
EC50 (μM) | 0.024 | 0.021 | 0.013 | |
Ligand | DHT | |||
Yeast strain | AR W303a | AR cwp1Δpdr5Δ | AR pdr5Δpdr10Δ | |
Fold difference (100 μM) | 1 | 14.4 | 18.6 | |
Detection limit (μM) | 0.01 | 0.01 | 0.01 | |
EC50 (μM) | 0.028 | 0.011 | 0.023 | |