Table 2 RT qPCR primers
Gene | Forward primer | Reverse primer |
|---|---|---|
MAGEA1 | caacccagaggacaggattc | gggcaatgaagacccaca |
MAGEA2 | atgaacgggctttgagagag | gaggcagtggaagctaatgg |
MAGEA3/A6 | gtgaggaggcaaggttctga | gggcaatggagacccact |
MAGEA4 | acagaggagcaccaaggaga | cagcaggcaagagtgcag |
MAGEA5 | agaggagcaccaaaggagaa | actctggtcaccgcaacag |
MAGEA8 | cactcctacatccttgtcacctg | ggtcttgggcgtactctgat |
MAGEA9 | ggagaggcctccttctgag | tctgcgacctgaggacact |
MAGEA10 | gttctgagggacaggcttga | gtcaccctctgagagcaagg |
MAGEA11 | tctttctgaggggtgtcttgag | gaactgagtctccatccctcag |
MAGEA12 | gattctcgccctgagcaa | gggcctgtctcctcagaac |
MAGEA1 (CDS) | gagtccttgttccgagcagt | aggagcagaaaaccaaccaa |
MAGEA2 (CDS) | caatagagggcgactgtgc | ccctcaaacacctccaacat |
MAGEA3/A6 (CDS) | aaggtggccaagttggttc | gcatttctgcctttgtgacc |
MAGEA4 (CDS) | cgcctctgaggaggaaatct | ccaatcttgggtgagcagtt |
MAGEA9 (CDS) | ttcatgcaggtgatctttgg | acgagaggccaagagcagt |
MAGEA10 (CDS) | gccactcctttgtccttgtc | gggcatgctctggacatc |
MAGEA11 (CDS) | gtgggtgcacaggctctc | gcccacattcagagtagagga |
MAGEA12 (CDS) | ctctctggagtcaatccgatg | caggtcaggaaaggtgcttg |
MAGED2 | ccagcaagatgaaagtcctca | tccatcgcctctcggtact |
RpLp (housekeeping gene) | tctacaaccctgaagtgcttgat | caatctgcagacagacactgg |