Fig. 3

Induction of OXR1 protein level. a, b OXR1 protein level. HeLa cells (indicated as HeLa WT) were treated with (a) 1.5 mM MMS for 1 h or (b) 2.5 Gy of carbon-ion beams, and the cells were incubated for the indicated times. The OXR1 protein level in crude extracts was assessed by Western blot using anti-OXR1 antibody. Proteins were separated with 10% SDS-PAGE gel. OXR1 protein was detected as (a) two or (b) single bands because of the difference in each gel running time. It has been confirmed that both two bands are OXR1 in our previous study [15]. Relative OXR1 protein levels (vs. no treatment) normalized by beta-actin or tubulin are shown below OXR1 blots. The size of marker proteins is shown on the right of the image. Images were analyzed by Image J software. NT, no treatment