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Fig. 1 | Genes and Environment

Fig. 1

From: Preparation of the standard cell lines for reference mutations in cancer gene-panels by genome editing in HEK 293 T/17 cells

Fig. 1

Results of T7E1 assay. After transfection of expression vectors pRGEN_U6_SG, pRGEN-Cas9-CMV and ssODN corresponding to the target genes (KRAS, NRAS, PIK3CA, PTEN, BRAF and TP53), genomic DNA was isolated from the transfected cells. The target region was amplified by PCA with corresponding primers. After denature and re-annealing of the PCR product, it was digested by T7 endonuclease I (T7E1) which cut mismatched DNA fragments. Cleaved band suggests an introduction of mutations by ssODN

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