Fig. 2From: Preparation of the standard cell lines for reference mutations in cancer gene-panels by genome editing in HEK 293 T/17 cellsConfirmation of the TP53 743 G > A mutation by Sanger sequencing. Existence of the targeted knock-in mutation was checked for each isolated clones by the Sanger sequencing after PCR amplification of the target region. Successful examples for TP53 743 G > A mutation are shown. Both homo and hetero knock-in mutants were obtainedBack to article page