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Table 2 Targetted mutations and recoveries of the knock-in mutants

From: Preparation of the standard cell lines for reference mutations in cancer gene-panels by genome editing in HEK 293 T/17 cells

Method Gene Mutation bp from PAM Total Clones Homo Knock-in Hetero Knock-in Targeted Rate(%)
Vector AKT1 49 G > A 1 177 0 0 0.0
Vector ALK 3824 G > A 4 190 4 5 6.8
Vector BAP1 178 C > T 5 183 0 12 6.6
Vector CDKN2A 238 C > T 2 156 5 2 7.7
Vector CTNNB1 121 A > G 6 164 1 0 1.2
Vector DNMT3A 2645 G > A 6 149 0 1 0.7
Vector ERBB3 310 G > A 4 171 0 1 0.6
Vector EZH2 1937 A > T 7 153 4 0 5.2
Vector FBXW7 1513 C > T 2 167 0 0 0.0
Vector FGFR3 746 C > G 1 167 0 1 0.6
Vector HRAS 183 G > T 2 172 2 5 5.2
Vector IDH2 515 G > A 2 155 0 7 4.5
Vector JAK2 1849 G > T 8 164 3 1 4.3
Vector KIT 2447 A > T 7 172 3 12 10.5
Vector KNSTRN 71 C > T 1 161 1 0 1.2
Vector KRAS 35 G > A 4 160 0 2 1.3
Vector MAGOH 410 T > C 4 160 0 5 3.1
Vector MAP 2 K1 370 C > T 1 177 1 0 1.1
Vector MAPK1 964 G > A 4 169 0 0 0.0
Vector MDM2 994 C > T 6 171 0 2 1.2
Vector MET 3029 C > T 5 154 1 1 1.9
Vector MTOR 6644 C > A 8 182 2 9 7.1
Vector NOTCH1 4799 T > C 9 167 0 2 1.2
Vector NRAS 35 G > A 1 157 2 0 2.5
Vector PDGFRA 2525 A > T 7 176 7 6 11.4
Vector PIK3CA 3140A > G 1 161 0 1 0.6
Vector PTEN 697 C > T 7 156 0 1 0.6
Vector SMO 1234 C > T 8 157 8 7 14.6
Vector STAT3 1919 A > T 5 161 0 1 0.6
Vector TP53 743 G > A 5 159 2 2 3.8
Protein BRAF 1817 G > A 1 172 1 16 10.5
Protein AKT3 232 C > A 2 192 0 5 2.6
Protein BIM 585 G > C 1 192 6 9 10.9
Protein IGF2 293 C > T 2 192 0 6 3.1
Protein MYCN 1132 G > A 1 192 10 26 24.0