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Table 2 Targetted mutations and recoveries of the knock-in mutants

From: Preparation of the standard cell lines for reference mutations in cancer gene-panels by genome editing in HEK 293 T/17 cells

Method

Gene

Mutation

bp from PAM

Total Clones

Homo Knock-in

Hetero Knock-in

Targeted Rate(%)

Vector

AKT1

49 G > A

1

177

0

0

0.0

Vector

ALK

3824 G > A

4

190

4

5

6.8

Vector

BAP1

178 C > T

5

183

0

12

6.6

Vector

CDKN2A

238 C > T

2

156

5

2

7.7

Vector

CTNNB1

121 A > G

6

164

1

0

1.2

Vector

DNMT3A

2645 G > A

6

149

0

1

0.7

Vector

ERBB3

310 G > A

4

171

0

1

0.6

Vector

EZH2

1937 A > T

7

153

4

0

5.2

Vector

FBXW7

1513 C > T

2

167

0

0

0.0

Vector

FGFR3

746 C > G

1

167

0

1

0.6

Vector

HRAS

183 G > T

2

172

2

5

5.2

Vector

IDH2

515 G > A

2

155

0

7

4.5

Vector

JAK2

1849 G > T

8

164

3

1

4.3

Vector

KIT

2447 A > T

7

172

3

12

10.5

Vector

KNSTRN

71 C > T

1

161

1

0

1.2

Vector

KRAS

35 G > A

4

160

0

2

1.3

Vector

MAGOH

410 T > C

4

160

0

5

3.1

Vector

MAP 2 K1

370 C > T

1

177

1

0

1.1

Vector

MAPK1

964 G > A

4

169

0

0

0.0

Vector

MDM2

994 C > T

6

171

0

2

1.2

Vector

MET

3029 C > T

5

154

1

1

1.9

Vector

MTOR

6644 C > A

8

182

2

9

7.1

Vector

NOTCH1

4799 T > C

9

167

0

2

1.2

Vector

NRAS

35 G > A

1

157

2

0

2.5

Vector

PDGFRA

2525 A > T

7

176

7

6

11.4

Vector

PIK3CA

3140A > G

1

161

0

1

0.6

Vector

PTEN

697 C > T

7

156

0

1

0.6

Vector

SMO

1234 C > T

8

157

8

7

14.6

Vector

STAT3

1919 A > T

5

161

0

1

0.6

Vector

TP53

743 G > A

5

159

2

2

3.8

Protein

BRAF

1817 G > A

1

172

1

16

10.5

Protein

AKT3

232 C > A

2

192

0

5

2.6

Protein

BIM

585 G > C

1

192

6

9

10.9

Protein

IGF2

293 C > T

2

192

0

6

3.1

Protein

MYCN

1132 G > A

1

192

10

26

24.0

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Genes and Environment

ISSN: 1880-7062

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