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Table 2 Targetted mutations and recoveries of the knock-in mutants

From: Preparation of the standard cell lines for reference mutations in cancer gene-panels by genome editing in HEK 293 T/17 cells

MethodGeneMutationbp from PAMTotal ClonesHomo Knock-inHetero Knock-inTargeted Rate(%)
VectorAKT149 G > A1177000.0
VectorALK3824 G > A4190456.8
VectorBAP1178 C > T51830126.6
VectorCDKN2A238 C > T2156527.7
VectorCTNNB1121 A > G6164101.2
VectorDNMT3A2645 G > A6149010.7
VectorERBB3310 G > A4171010.6
VectorEZH21937 A > T7153405.2
VectorFBXW71513 C > T2167000.0
VectorFGFR3746 C > G1167010.6
VectorHRAS183 G > T2172255.2
VectorIDH2515 G > A2155074.5
VectorJAK21849 G > T8164314.3
VectorKIT2447 A > T717231210.5
VectorKNSTRN71 C > T1161101.2
VectorKRAS35 G > A4160021.3
VectorMAGOH410 T > C4160053.1
VectorMAP 2 K1370 C > T1177101.1
VectorMAPK1964 G > A4169000.0
VectorMDM2994 C > T6171021.2
VectorMET3029 C > T5154111.9
VectorMTOR6644 C > A8182297.1
VectorNOTCH14799 T > C9167021.2
VectorNRAS35 G > A1157202.5
VectorPDGFRA2525 A > T71767611.4
VectorPIK3CA3140A > G1161010.6
VectorPTEN697 C > T7156010.6
VectorSMO1234 C > T81578714.6
VectorSTAT31919 A > T5161010.6
VectorTP53743 G > A5159223.8
ProteinBRAF1817 G > A117211610.5
ProteinAKT3232 C > A2192052.6
ProteinBIM585 G > C11926910.9
ProteinIGF2293 C > T2192063.1
ProteinMYCN1132 G > A1192102624.0