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Table 3 List of Mutant Cell Lines Created by Genome Editting in HEK293T/17 Cell

From: Preparation of the standard cell lines for reference mutations in cancer gene-panels by genome editing in HEK 293 T/17 cells

Target Gene Cell Clone Name (ID) JCRB Cell ID Mutation detaila
AKT1 49 G>A 293T-AKT1-1 delTG(43-44) Homo
293T-AKT1-2 49G>A(COSM33765)hetero+55insT&49G>A(COSM33765)Hetero
AKT3 232C>A 293T-AKT3-1 232C>A(COSM242892)Hetero+del231-238(8bp) Hetero+236delG&235T>A Hetero
293T-AKT3-2 232C>A(COSM242892)Hetero
ALK 3824 G>A 293T-ALK-1 3824G>A(COSM28056)Homo
293T-ALK-2 3824G>A(COSM28056)Hetero
BAP1 178 C>T 293T-BAP1-1 178C>T(COSM110721)Hetero
293T-BAP1-2 178C>T(COSM110721)Hetero&160-173(14bp) duplicate
BIM 585G>C 293T-BIM-1 585G>C(COSM389356)Homo+590 insTHetero
293T-BIM-2 585G>C(COSM389356)Hetero
BRAF 1817G>A 293T-BRAF-1 1817G>A(COSM1137)Hetero
293T-BRAF-2 1817G>A(COSM1137)Hetero
293T-BRAF-3 1817G>A(COSM1137)Hetero+1820delC&1817G>A(COSM1137)Hetero
293T-BRAF-4 1817G>A(COSM1137)Hetero+1811insG Hetero
293T-BRAF-5 1817G>A(COSM1137)Homo
CDKN2A 238 C>T 293T-CDKN2A-1 238C>T(COSM12475)Hetero
293T-CDKN2A-2 238C>T(COSM12475)Homo
CTNNB1 121 A>G 293T-CTNNB1-1 121A>G(COSM5664)Homo+del116-9&112G>A Hetero+del114-9 Hetero
293T-CTNNB1-2 121A>G(COSM5664)Homo
DNMT3A 2645 G>A 293T-DNMT3A-1 2643insC Homo
293T-DNMT3A-2 2645G>A(COSM52944)Hetero
ERBB3 310 G>A 293T-ERBB3-1 306insC Homo
293T-ERBB3-2 310G>A(COSM20710)Hetero
EZH2 1937 A>T 293T-EZH2-1 1937A>T(COSM37028)Homo+1831T>C Homo
293T-EZH2-2 1937A>T(COSM37028)Homo
FBXW7 1513 C>T 293T-FBXW7-1 1518insT Homo
293T-FBXW7-2 1518insT Hetero+1518insCA Hetero
FGFR3 746 C>G 293T-FGFR3-1 752insA Homo
293T-FGFR3-2 746C>G(COSM715)Hetero
HRAS 183 G>T 293T-HRAS-1 183G>T(COSM502)Homo
293T-HRAS-2 183G>T(COSM502)Hetero
IDH2 515 G>A 293T-IDH2-1 515G>A(COSM33733)Hetero+512G>A(COSM86960) Hetero
293T-IDH2-2 515G>A(COSM33733)Hetero
IGF2 293C>T 293T-IGF2-1 293C>T(COSM1561457)Hetero&del276-289(14bp)Hetero +294-295insCA+del270-283(14bp)Hetero
293T-IGF2-2 293C>T(COSM1561457)Hetero
JAK2 1849 G>T 293T-JAK2-1 1849G>T(COSM12600)Homo
293T-JAK2-2 1849G>T(COSM12600)Hetero
KIT 2447 A>T 293T-KIT-1 2447A>T(COSM1314)Homo
293T-KIT-2 2447A>T(COSM1314)Hetero
KNSTRN 71 C>T 293T-KNSTRN-1 71C>T(COSM140056) Homo
293T-KNSTRN-2 71C>T(COSM140056) &Del67-69(3bp) Homo
KRAS 35 G>A 293T-KRAS-1 32insC Homo+35G>A(COSM521)Hetero+del30-38(9bp) Hetero
293T-KRAS-2 35G>A(COSM521)Hetero+33delT Hetero
293T-KRAS-3 35G>A(COSM521)Hetero
293T-KRAS-4 35G>A(COSM521)&30-33AGCT>CGTA Hetero+32insC Hetero
293T-KRAS-5 35G>A(COSM521)&del20-34(15bp)Hetero+32insCC Hetero+32insC Hetero
MAGOH 410 T>C 293T-MAGOH-1 410T>C(COSM535605)Hetero
293T-MAGOH-2 410T>C(COSM535605)&407delT Hetero
MAP2K1 370 C>T 293T-MAP2K1-1 370C>T(COSM235614)Hetero+370C>T(COSM235614)Hetero&376insA hetero+358-377(20bp) duplicate Hetero
293T-MAP2K1-2 376insA Homo
MAPK1 964 G>A 293T-MAPK1-1 960insG Homo
293T-MAPK1-2 del962-968(7bp) Hetero+del962-969&4bp(12bp) Hetero+del926-969&69bp(113bp) Hetero
MDM2 994 C>T 293T-MDM2-1 995insG Homo
293T-MDM2-2 994C>T(COSM431747)Hetero
MET 3029 C>T 293T-MET-1 3029C>T(COSM707)Hetero
293T-MET-2 3029C>T(COSM707)Homo
MTOR 6644 C>A 293T-MTOR-1 6644C>A(COSM20417)Hetero
293T-MTOR-2 6644C>A(COSM20417)Homo
MYCN 1132 G>A 293T-MYCN-1 1132G>A(COSM229914)Homo
293T-MYCN-2 1132G>A(COSM229914)Hetero+1126delG Hetero
NOTCH1 4799 T>C 293T-NOTCH1-1 4799T>C(COSM12771)Hetero
293T-NOTCH1-2 4797insG Homo
NRAS 35 G>A 293T-NRAS-1 35G>A(COSM564)Homo
293T-NRAS-2 35G>A(COSM564)Homo
293T-NRAS-3 34G>T(COSM562)Hetero
293T-NRAS-4 35G>A(COSM564)Hetero+30-31AG>GAAA Hetero
293T-NRAS-5 35G>A(COSM564)Hetero+del30-44(15bp) Hetero
PDGFRA 2525 A>T 293T-PDGFRA-1 2525A>T(COSM736)Homo
293T-PDGFRA-2 2525A>T(COSM736)Hetero
PIK3CA 3140A>G 293T-PIK3CA-1 3140A>G(COSM775)Hetero+3143insA Hetero
293T-PIK3CA-2 3144T>G(COSM27157)Homo
293T-PIK3CA-3 3140A>G(COSM775)Hetero+del3131-42(12bp) Hetero
293T-PIK3CA-4 3140A>G(COSM775)Hetero
293T-PIK3CA-5 3140A>G(COSM775)Hetero+del3102-47(46bp) Hetero
PTEN 697 C>T 293T-PTEN-1 63-64 CG>(T,C,G)(T,C,G)(A,G) (ins 1bp for 3 alleles)
293T-PTEN-2 697C>T(COSM5154)Hetero
293T-PTEN-3 697C>T(COSM5154)Hetero+697insA Hetero+697-698CG>TT Hetero
293T-PTEN-4 697insGorA Hetero
293T-PTEN-5 697C>T(COSM5154)Hetero&Del 546-672(127bp)&546 5bp ins(535-539) & Del697-715(19bp) Hetero +WT
SMO 1234C>T 293T-SMO-1 1234C>T(COSM216037)Homo
293T-SMO-2 1234C>T(COSM216037)Hetero
STAT3 1919 A>T 293T-STAT3-1 1921insA Homo
293T-STAT3-2 1919A>T(COSM1155743)Hetero
TP53 743 G>A 293T-TP53-1 743G>A(COSM10662)Homo+746G>A(COSM44091)Homo
293T-TP53-2 743G>A(COSM10662)Hetero
293T-TP53-3 743G>A(COSM10662)Homo
293T-TP53-4 743G>A(COSM10662)Homo
293T-TP53-5 743G>A(COSM10662)&746delG Homo
  1. a& means mutations were found in the same strand, + for other cases