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Table 3 List of Mutant Cell Lines Created by Genome Editting in HEK293T/17 Cell

From: Preparation of the standard cell lines for reference mutations in cancer gene-panels by genome editing in HEK 293 T/17 cells

Target GeneCell Clone Name (ID)JCRB Cell IDMutation detaila
AKT149 G>A293T-AKT1-1delTG(43-44) Homo
293T-AKT1-249G>A(COSM33765)hetero+55insT&49G>A(COSM33765)Hetero
AKT3232C>A293T-AKT3-1232C>A(COSM242892)Hetero+del231-238(8bp) Hetero+236delG&235T>A Hetero
293T-AKT3-2232C>A(COSM242892)Hetero
ALK3824 G>A293T-ALK-13824G>A(COSM28056)Homo
293T-ALK-23824G>A(COSM28056)Hetero
BAP1178 C>T293T-BAP1-1178C>T(COSM110721)Hetero
293T-BAP1-2178C>T(COSM110721)Hetero&160-173(14bp) duplicate
BIM585G>C293T-BIM-1585G>C(COSM389356)Homo+590 insTHetero
293T-BIM-2585G>C(COSM389356)Hetero
BRAF1817G>A293T-BRAF-11817G>A(COSM1137)Hetero
293T-BRAF-21817G>A(COSM1137)Hetero
293T-BRAF-31817G>A(COSM1137)Hetero+1820delC&1817G>A(COSM1137)Hetero
293T-BRAF-41817G>A(COSM1137)Hetero+1811insG Hetero
293T-BRAF-51817G>A(COSM1137)Homo
CDKN2A238 C>T293T-CDKN2A-1238C>T(COSM12475)Hetero
293T-CDKN2A-2238C>T(COSM12475)Homo
CTNNB1121 A>G293T-CTNNB1-1121A>G(COSM5664)Homo+del116-9&112G>A Hetero+del114-9 Hetero
293T-CTNNB1-2121A>G(COSM5664)Homo
DNMT3A2645 G>A293T-DNMT3A-12643insC Homo
293T-DNMT3A-22645G>A(COSM52944)Hetero
ERBB3310 G>A293T-ERBB3-1306insC Homo
293T-ERBB3-2310G>A(COSM20710)Hetero
EZH21937 A>T293T-EZH2-11937A>T(COSM37028)Homo+1831T>C Homo
293T-EZH2-21937A>T(COSM37028)Homo
FBXW71513 C>T293T-FBXW7-11518insT Homo
293T-FBXW7-21518insT Hetero+1518insCA Hetero
FGFR3746 C>G293T-FGFR3-1752insA Homo
293T-FGFR3-2746C>G(COSM715)Hetero
HRAS183 G>T293T-HRAS-1183G>T(COSM502)Homo
293T-HRAS-2183G>T(COSM502)Hetero
IDH2515 G>A293T-IDH2-1515G>A(COSM33733)Hetero+512G>A(COSM86960) Hetero
293T-IDH2-2515G>A(COSM33733)Hetero
IGF2293C>T293T-IGF2-1293C>T(COSM1561457)Hetero&del276-289(14bp)Hetero +294-295insCA+del270-283(14bp)Hetero
293T-IGF2-2293C>T(COSM1561457)Hetero
JAK21849 G>T293T-JAK2-11849G>T(COSM12600)Homo
293T-JAK2-21849G>T(COSM12600)Hetero
KIT2447 A>T293T-KIT-12447A>T(COSM1314)Homo
293T-KIT-22447A>T(COSM1314)Hetero
KNSTRN71 C>T293T-KNSTRN-171C>T(COSM140056) Homo
293T-KNSTRN-271C>T(COSM140056) &Del67-69(3bp) Homo
KRAS35 G>A293T-KRAS-132insC Homo+35G>A(COSM521)Hetero+del30-38(9bp) Hetero
293T-KRAS-235G>A(COSM521)Hetero+33delT Hetero
293T-KRAS-335G>A(COSM521)Hetero
293T-KRAS-435G>A(COSM521)&30-33AGCT>CGTA Hetero+32insC Hetero
293T-KRAS-535G>A(COSM521)&del20-34(15bp)Hetero+32insCC Hetero+32insC Hetero
MAGOH410 T>C293T-MAGOH-1410T>C(COSM535605)Hetero
293T-MAGOH-2410T>C(COSM535605)&407delT Hetero
MAP2K1370 C>T293T-MAP2K1-1370C>T(COSM235614)Hetero+370C>T(COSM235614)Hetero&376insA hetero+358-377(20bp) duplicate Hetero
293T-MAP2K1-2376insA Homo
MAPK1964 G>A293T-MAPK1-1960insG Homo
293T-MAPK1-2del962-968(7bp) Hetero+del962-969&4bp(12bp) Hetero+del926-969&69bp(113bp) Hetero
MDM2994 C>T293T-MDM2-1995insG Homo
293T-MDM2-2994C>T(COSM431747)Hetero
MET3029 C>T293T-MET-13029C>T(COSM707)Hetero
293T-MET-23029C>T(COSM707)Homo
MTOR6644 C>A293T-MTOR-16644C>A(COSM20417)Hetero
293T-MTOR-26644C>A(COSM20417)Homo
MYCN1132 G>A293T-MYCN-11132G>A(COSM229914)Homo
293T-MYCN-21132G>A(COSM229914)Hetero+1126delG Hetero
NOTCH14799 T>C293T-NOTCH1-14799T>C(COSM12771)Hetero
293T-NOTCH1-24797insG Homo
NRAS35 G>A293T-NRAS-135G>A(COSM564)Homo
293T-NRAS-235G>A(COSM564)Homo
293T-NRAS-334G>T(COSM562)Hetero
293T-NRAS-435G>A(COSM564)Hetero+30-31AG>GAAA Hetero
293T-NRAS-535G>A(COSM564)Hetero+del30-44(15bp) Hetero
PDGFRA2525 A>T293T-PDGFRA-12525A>T(COSM736)Homo
293T-PDGFRA-22525A>T(COSM736)Hetero
PIK3CA3140A>G293T-PIK3CA-13140A>G(COSM775)Hetero+3143insA Hetero
293T-PIK3CA-23144T>G(COSM27157)Homo
293T-PIK3CA-33140A>G(COSM775)Hetero+del3131-42(12bp) Hetero
293T-PIK3CA-43140A>G(COSM775)Hetero
293T-PIK3CA-53140A>G(COSM775)Hetero+del3102-47(46bp) Hetero
PTEN697 C>T293T-PTEN-163-64 CG>(T,C,G)(T,C,G)(A,G) (ins 1bp for 3 alleles)
293T-PTEN-2697C>T(COSM5154)Hetero
293T-PTEN-3697C>T(COSM5154)Hetero+697insA Hetero+697-698CG>TT Hetero
293T-PTEN-4697insGorA Hetero
293T-PTEN-5697C>T(COSM5154)Hetero&Del 546-672(127bp)&546 5bp ins(535-539) & Del697-715(19bp) Hetero +WT
SMO1234C>T293T-SMO-11234C>T(COSM216037)Homo
293T-SMO-21234C>T(COSM216037)Hetero
STAT31919 A>T293T-STAT3-11921insA Homo
293T-STAT3-21919A>T(COSM1155743)Hetero
TP53743 G>A293T-TP53-1743G>A(COSM10662)Homo+746G>A(COSM44091)Homo
293T-TP53-2743G>A(COSM10662)Hetero
293T-TP53-3743G>A(COSM10662)Homo
293T-TP53-4743G>A(COSM10662)Homo
293T-TP53-5743G>A(COSM10662)&746delG Homo
  1. a& means mutations were found in the same strand, + for other cases
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