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Table 2 PCR conditions used for polymorphisms studied

From: Radio-adaptive response and correlation of non-homologous end joining repair gene polymorphisms [XRRC5 (3R/2R/1R/0R), XRCC6(C/G) and XRCC7 (G/T)] in human peripheral blood mononuclear cells exposed to gamma radiation

 

XRCC5

(KU80)

XRCC6

(KU70)

XRCC7

(PRKDC)

Temp

Time

Temp

Time

Temp

Time

Initial denaturation

95 °C

5 min

95 °C

5 min

95 °C

5 min

Amplification (30 cycles)

Annealing

60 °C

30s

58 °C

30s

63 °C

30s

Extension

72 °C

30s

72 °C

30s

72 °C

30s

Denaturation

95 °C

30s

95 °C

30s

95 °C

30s

Final extension

 

72 °C

5 min

72 °C

5 min

72 °C

5 min

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Genes and Environment

ISSN: 1880-7062

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