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Table 2 PCR conditions used for polymorphisms studied

From: Radio-adaptive response and correlation of non-homologous end joining repair gene polymorphisms [XRRC5 (3R/2R/1R/0R), XRCC6(C/G) and XRCC7 (G/T)] in human peripheral blood mononuclear cells exposed to gamma radiation

  XRCC5
(KU80)
XRCC6
(KU70)
XRCC7
(PRKDC)
Temp Time Temp Time Temp Time
Initial denaturation 95 °C 5 min 95 °C 5 min 95 °C 5 min
Amplification (30 cycles) Annealing 60 °C 30s 58 °C 30s 63 °C 30s
Extension 72 °C 30s 72 °C 30s 72 °C 30s
Denaturation 95 °C 30s 95 °C 30s 95 °C 30s
Final extension   72 °C 5 min 72 °C 5 min 72 °C 5 min