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Table 1 The amplification protocols for detection of the ACE I/D and ACTN3 polymorphisms

From: The ACE and ACTN3 polymorphisms in female soccer athletes

 

ACE I/D

ACTN3

Protocol

denaturation

95℃

3 min

denaturation

95℃

3 min

denaturation

95℃

30s

denaturation

95℃

30s

annealing

60℃

30s

annealing

68℃

30s

extension

72℃

8 min

extension

68℃

8 min