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Table 1 The amplification protocols for detection of the ACE I/D and ACTN3 polymorphisms

From: The ACE and ACTN3 polymorphisms in female soccer athletes

  ACE I/D ACTN3
Protocol denaturation 95℃ 3 min denaturation 95℃ 3 min
denaturation 95℃ 30s denaturation 95℃ 30s
annealing 60℃ 30s annealing 68℃ 30s
extension 72℃ 8 min extension 68℃ 8 min