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Table 3 Summary of results of TK gene mutation assay for the 10 chemicals

From: Weight of evidence approach using a TK gene mutation assay with human TK6 cells for follow-up of positive results in Ames tests: a collaborative study by MMS/JEMS

No.

Chemical Name

CAS No.

TK mutation assaya

Bacterial reverse mutation assayb

MLA (TK gene locus)

Without S9

With S9

Long term

Without S9

With S9

Without S9

With S9

Ref.

1

4-(Chloroacetyl)-acetanilide

140-49-8

Pos

Neg

Pos

Neg

Pos

No data

 

2

2-(Chloromethyl) pyridine HCl

6959-47-3

Pos

Pos

NP

Pos

Pos

Pos

Pos

[47]

3

2,6-Diaminotoluene

823-40-5

Neg

Pos

Pos

Neg

Pos

Pos

No data

[48]

4

2,5-Diaminotoluene

95-70-5

Neg

Neg

Neg

Neg

Pos

Inconclusivec

[46, 49]

5

HC Blue No.2

33229-34-4

Neg

Neg

Pos

Pos

Pos

Pos

Pos

[27]

6

8-Hydroxyquinoline

148-24-3

Neg

Pos

NP

Neg

Pos

Pos

No data

[50]

7

Iodoform

75-47-8

Neg

Neg

Neg

Pos

Pos

No data

 

8

4-Nitroanthranilic acid

619-17-0

Neg

Neg

Pos

Pos

Pos

Equivocald

Pos

[51]

9

1-Nitronaphthalene

86-57-7

Pos

Pos

NP

Pos

Pos

No data

 

10

4-Nitro-o-phenylenediamine

99-56-9

Pos

Neg

Pos

Pos

Pos

Pos

Pos

[52, 53]

  1. aPerformed in this study. NP Not applicated
  2. bTaken from Table 2
  3. cExamined as toluene-2,5-diamine sulfate. The required toxicity (10–20% survival compared to the concurrent negative controls) was not reached in the experiments with S9mix [46]
  4. dSignificant difference only at the highest dose of 1200 μg/mL [51]