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Fig. 1 | Genes and Environment

Fig. 1

From: Standard protocol for the PIGRET assay, a high-throughput reticulocyte Pig-a assay with an immunomagnetic separation, used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen and Genome Society

Fig. 1

Outline of the PIGRET assay procedure. First, stain RBCs with PE-conjugated anti-CD71 Abs and mixed with anti-PE magnet particles. Enrich CD71 positive reticulocytes using cell separation magnet(s) by a positive selection method. Next, stain the enriched RETs with APC-conjugated anti-HIS49 Ab and FITC-conjugated anti-CD59 Ab. Finally, using FCM, CD71 and HIS49 double-positive cells are analyzed for CD59 expression, and Pig-a mutant cells are detected as the FITC-negative population

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Genes and Environment

ISSN: 1880-7062

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