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Fig. 2 | Genes and Environment

Fig. 2

From: Standard protocol for the PIGRET assay, a high-throughput reticulocyte Pig-a assay with an immunomagnetic separation, used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen and Genome Society

Fig. 2

Plots for the PIGRET assay. Create dot-plots of FSC vs. SSC (Plot 1), PE vs. APC (Plot 3), FSC vs. FITC (Plot 4), and PE vs. FITC (Plot 5). Create one more dot-plot of FSC-H/FSC-W to eliminate doublet cells (Plot 2, option for digital FCM). Adjust or create the “Pig-a mutants” region to include 99.0% ± 0.1% of the CD59-negative cell population using the HIS49 single-stain sample

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Genes and Environment

ISSN: 1880-7062

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