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Fig. 4 | Genes and Environment

Fig. 4

From: Standard protocol for the PIGRET assay, a high-throughput reticulocyte Pig-a assay with an immunomagnetic separation, used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen and Genome Society

Fig. 4

Gate defining and adjustment for Pig-a mutants with HIS49 single-stain samples (copy-and-paste method for FACSCantoII). Create “temporary Pig-a mutants” gate in Plot 4. Adjust the height of the “temporary Pig-a mutants” gate to include 99.0% ± 0.1% of the CD59-negative cell population using the HIS49 single-stain sample. Make sure that the “temporary Pig-a mutants” gate has reached the x-axis. If applicable, select “Bi-exponential” for the y-axis (FITC intensity scaling) in the Plot Inspector. Then, copy-paste the temporary gate so that the gate would be created as a subset of “CD71 + HIS49 + .” Rename to “Pig-a Mutants” and change Show Populations back to the “CD71 + HIS49+” population. The marker of “temporary Pig-a mutants” can be hidden if appropriate

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Genes and Environment

ISSN: 1880-7062

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