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Table 1 Mean % tail DNA and hedgehog frequency in the corneal epithelial cells

From: In vivo comet assay in rabbit corneal epithelial cells following ocular instillation with genotoxic compounds

Compounds

Concentration

Number of eyes

% tail DNA

(Mean ± SD)

Hedgehog (%)

(Mean ± SD)

Test 1

 Saline

0 %

3

7.3

±

0.4

0.0

±

0.0

 Ethidium bromide

0.25 %

3

15.2

±

1.9a

0.7

±

1.2

 

1 %

3

24.0

±

1.6a

7.0

±

5.6

 Paraquat

1 %

3

8.0

±

2.5

0.0

±

0.0

 

5 %

3

6.8

±

1.2

0.0

±

0.0

Test 2

 Saline

0 %

3

8.8

±

2.2

0.0

±

0.0

 MMS

0.6 %

3

39.9

±

6.0a

0.0

±

0.0

 

3 %

3

55.1

±

3.9a

7.3

±

4.6

 Acrylamide

0.6 %

3

10.3

±

5.2

0.0

±

0.0

 

3 %

3

12.1

±

1.7

0.0

±

0.0

Test 3

 5 % DMSO

0 %

3

7.9

±

2.0

0.0

±

0.0

 4-NQO

0.2 %

3

24.6

±

3.2a

1.3

±

1.2

 

1 %

3

36.0

±

11.6a

6.7

±

4.2

  1. a: Significantly higher than the negative control at the probability of 5 % level (Dunnett’s multiple comparison test, one-tailed)
  2. Paraquat: 1,1′-dimethyl-4,4′-bipyridinium dichloride, MMS: methyl methanesulfonate, 5 % DMSO: 5 % dimethyl sulfoxide in saline, 4-NQO: 4-nitroquinoline 1-oxide