In vivo comet assay in rabbit corneal epithelial cells following ocular instillation with genotoxic compounds

Genes and Environment

Table 1 Mean % tail DNA and hedgehog frequency in the corneal epithelial cells

Compounds	Concentration	Number of eyes	% tail DNA (Mean ± SD)			Hedgehog (%) (Mean ± SD)
Test 1
Saline	0 %	3	7.3	±	0.4	0.0	±	0.0
Ethidium bromide	0.25 %	3	15.2	±	1.9^a	0.7	±	1.2
	1 %	3	24.0	±	1.6^a	7.0	±	5.6
Paraquat	1 %	3	8.0	±	2.5	0.0	±	0.0
	5 %	3	6.8	±	1.2	0.0	±	0.0
Test 2
Saline	0 %	3	8.8	±	2.2	0.0	±	0.0
MMS	0.6 %	3	39.9	±	6.0^a	0.0	±	0.0
	3 %	3	55.1	±	3.9^a	7.3	±	4.6
Acrylamide	0.6 %	3	10.3	±	5.2	0.0	±	0.0
	3 %	3	12.1	±	1.7	0.0	±	0.0
Test 3
5 % DMSO	0 %	3	7.9	±	2.0	0.0	±	0.0
4-NQO	0.2 %	3	24.6	±	3.2^a	1.3	±	1.2
	1 %	3	36.0	±	11.6^a	6.7	±	4.2

^a: Significantly higher than the negative control at the probability of 5 % level (Dunnett’s multiple comparison test, one-tailed)
Paraquat: 1,1′-dimethyl-4,4′-bipyridinium dichloride, MMS: methyl methanesulfonate, 5 % DMSO: 5 % dimethyl sulfoxide in saline, 4-NQO: 4-nitroquinoline 1-oxide

ISSN: 1880-7062