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Table 1 Mean % tail DNA and hedgehog frequency in the corneal epithelial cells

From: In vivo comet assay in rabbit corneal epithelial cells following ocular instillation with genotoxic compounds

Compounds Concentration Number of eyes % tail DNA
(Mean ± SD)
Hedgehog (%)
(Mean ± SD)
Test 1
 Saline 0 % 3 7.3 ± 0.4 0.0 ± 0.0
 Ethidium bromide 0.25 % 3 15.2 ± 1.9a 0.7 ± 1.2
  1 % 3 24.0 ± 1.6a 7.0 ± 5.6
 Paraquat 1 % 3 8.0 ± 2.5 0.0 ± 0.0
  5 % 3 6.8 ± 1.2 0.0 ± 0.0
Test 2
 Saline 0 % 3 8.8 ± 2.2 0.0 ± 0.0
 MMS 0.6 % 3 39.9 ± 6.0a 0.0 ± 0.0
  3 % 3 55.1 ± 3.9a 7.3 ± 4.6
 Acrylamide 0.6 % 3 10.3 ± 5.2 0.0 ± 0.0
  3 % 3 12.1 ± 1.7 0.0 ± 0.0
Test 3
 5 % DMSO 0 % 3 7.9 ± 2.0 0.0 ± 0.0
 4-NQO 0.2 % 3 24.6 ± 3.2a 1.3 ± 1.2
  1 % 3 36.0 ± 11.6a 6.7 ± 4.2
  1. a: Significantly higher than the negative control at the probability of 5 % level (Dunnett’s multiple comparison test, one-tailed)
  2. Paraquat: 1,1′-dimethyl-4,4′-bipyridinium dichloride, MMS: methyl methanesulfonate, 5 % DMSO: 5 % dimethyl sulfoxide in saline, 4-NQO: 4-nitroquinoline 1-oxide