Table 1 Description and primers for 4 SNPs within VDR gene used for PCR analysis

FokI rs2228570

12:47,879,112

Missense

72.5 °C

C > T

FokI

F: GCACTGACTCTGGCTCTGAC

R: ACCCTCCTGCTCCTGTGGCT

ApaI

rs7975232

12:47,845,054

Intron variant

66.0 °C

C > A

ApaI

F: CAGAGCATGGACAGGGAGCAA

R: GCAACTCCTCATGGCTGAGGTCTC

TaqI rs731236

12:47,844,974

Coding sequence variant, synonymous variant

51.9 °C

T > C

TaqI

F:5’- CCTGTGCCTTCTTCTCTAT − 3’

R: 5’- CTAGCTTCTGGATCATCTTG- 3’

BsmI rs1544410

12:47,846,052

Intron variant

50.5 °C

G > A

BsmFI

F: 5’- ATATAGGCAGAACCATCTCT − 3’

R: 5’- TCTGAGGAACTAGATAAGCA − 3’